中国病理生理杂志2001，Vol.17Issue(2)：150-153,4.
ATP及其衍生物影响不死化人成纤维细胞增殖的实验研究
Experimental study on effects of ATP and its derivatives on the proliferation of the immortalized human fibroblasts
李君武1
作者信息
- 1. 暨南大学医学院微生物学免疫学教研室， 广东 广州 510632
- 折叠
摘要
Abstract
AIM： To investigate the inhibitory effects of ATP onproliferation of immortalized human fibroblasts, and learn what subtypes of P-2 purinergic receptors are involved in ATP cytotoxicity. METHODS:The immortalized human fibroblast cell line KMST-6 was cultured with ATP and its derivatives including ADP, β,γ-methyleneadenosine5'-triphosphate(MeATP), 2'&3'-O-(4-benzoylbenzoyl) adenosine, triethylammonium salt (BzATP), adenosine 5'-O-(3-methiotriphosphate)(ATPγS), 2-methylthioadenosine 5'-triphosphate(2-MeATP) and UTP, and the cell cycle analysis was finished using flow-cytometer, the cell apoptosis analysis was made using Hoechst 33258 and examination of DNA ladder. RESULTS： The extent of cytotoxicity induced by these drugs was found to be in order of : ATP=ADP＞ATPγS＞MeATP=BzATP, neither 2-MeSATP nor UTP showed any cytotoxicity. No enhanced expression of P21 was observed and the cell cycle was held in G1/S in the cells treated with 0.4mmol/L；no cell apoptosis was detected in the cells treated with 1mmol/L ATP. CONCLUSION： Connecting with P2X or P2Y purinergic receptors，ATP activated some intracellular signals to inhibit cell growth, the growth inhibition caused by ATP was not due to apoptosis or induction of cyclin/CDK kinase inhibitor,P21.关键词
腺苷三磷酸/细胞/受体,嘌呤能分类
生物科学引用本文复制引用
李君武..ATP及其衍生物影响不死化人成纤维细胞增殖的实验研究[J].中国病理生理杂志,2001,17(2):150-153,4.
