中华医学杂志（英文版）2002，Vol.115Issue(10)：1507-1509,3.
检测人巨细胞病毒晚期mRNA在诊断宫内活动性感染的价值
Diagnostic value of human cytomegalovirus late mRNA detection in active intrauterine infection
摘要
Abstract
Objective To study the diagnostic value of human cytomegalovirus (HCMV) late mRNA detection in active intrauterine infection.Methods The HCMV late mRNA in peripheral blood of 42 HCMV IgM positive pregnant women an d their fetal attachments (such as chorionic villi, amniotic fluid, umbilical bl ood and placenta) were detected by reverse transcription polymerase chain reacti on (RT-PCR).Results Late mRNA was detected in 23 of 42 HCMV IgM positive cases, a rate of 54.3%. Fetal appendages in 13 cases of late mRNA positive mothers were also tested, of which 7 were positive, with a vertical transmission rate of 53.8%. In 12 late m RNA negative mothers, only 1 case of fetal appendages tested was positive, with a vertical transmission rate of 8.3%. There was significant difference between the transmission rates of these two groups.Conclusions Positive results of HCMV IgM cannot accurately reflect the activity of HCMV at t he time of testing. However, the activity of HCMV is closely related to the mot her-fetus vertical transmission rate. As an indicator of active HCMV infection, late mRNA can not only reflect the mother-fetus transmission rate during active HCMV infection, but also provide some information about HCMV activity in fetal tissue.关键词
人巨细胞病毒 mRNA 宫内感染Key words
human cytomegalovirus/mRNA/intrauterine infection分类
医药卫生引用本文复制引用
姜宏,闻良珍,凌霞珍..检测人巨细胞病毒晚期mRNA在诊断宫内活动性感染的价值[J].中华医学杂志（英文版）,2002,115(10):1507-1509,3.基金项目
This work was supported by a grant from the"95" Key Research Program of China ( No. 96-904-06-08). （ No. 96-904-06-08）
