生殖医学杂志2006，Vol.15Issue(z1)：15-17,3.
Inhibin alpha gene G769A mutation in Chinese women with premature ovarian failure
Inhibin alpha gene G769A mutation in Chinese women with premature ovarian failure
Chen Xin-na 1Chen Gui-an 1Li Mei-zhi1
作者信息
- 1. Reproductive Medicine Center, Department of Obstetrics & Gynecology, Pekjing University Third Hospital, Beijing 100083, China
- 折叠
摘要
Abstract
Objective: To examine whether the inhibin alpha (INHα) gene G769A mutation is present in Chinese women with idiopathic premature ovarian failure (POF). Methods: The study was carried out in 77 Chinese women with idiopathic POF and 35 normal controls(including 25 normal females with a regular menstrual history and 10 normal post-menopause women)by a case-control analysis. Genomic DNA was extracted from the peripheral blood of the patients and control subjects. The inhibin alpha gene was amplified by PCR. The PCR products were subsequently digested with enzyme BbvI, and then were subjected to electrophoresis on agarose gels and stained with ethidium bromide to determine the INHα G769A mutation. Results:With BbvI digestion three fragments of 130, 88 and 25 base pairs were noted for all 77 POF patiens and 35 controls, thus demonstrating normal inhibin alpha allele. No patient or control was heterozygous or homozygous for the mutant allele. Conclusions: The inhibin alpha gene mutation may be rare in Chinese women with POF. The etiology of idiopathic POF for most patients deserves further investigation.关键词
Premature ovarian failure/Inhibin/MutationKey words
Premature ovarian failure/Inhibin/Mutation分类
医药卫生引用本文复制引用
Chen Xin-na,Chen Gui-an,Li Mei-zhi..Inhibin alpha gene G769A mutation in Chinese women with premature ovarian failure[J].生殖医学杂志,2006,15(z1):15-17,3.
