华西医科大学学报2001，Vol.32Issue(1)：15-17,3.
血链球菌丙酮酸氧化酶基因表达克隆的构建与鉴定
Expression of Pyruvate Oxidase Gene Sopox from Streptococcus Sanguis in E. coli
摘要
Abstract
Objective To reconstruct the expression plasmid of Sopox gene forfurther understanding the regulation of its expression. Methods Sopox was recombined with expression vector pBV220 and the expression of Sopox in E. coli JM105 was observed after transformation. Results pBV220/Sopox/JM105 expressed a protein with molecular weight of 65 kd on SDS-PAGE after induction, and the expression reached the maximal amount with induction at 42℃ for 4 hours. Conclusion Sopox was successfully cloned into pBV220 and expressed in E.coli JM105.关键词
丙酮酸氧化酶/血链球菌/基因表达分类
医药卫生引用本文复制引用
侯本祥,章锦才,张蓉,张蕴惠..血链球菌丙酮酸氧化酶基因表达克隆的构建与鉴定[J].华西医科大学学报,2001,32(1):15-17,3.基金项目
国家教委跨世纪人才培养计划基金 （）
