中国当代儿科杂志2004，Vol.6Issue(1)：15-18,4.
四硫化四砷STI571和除莠霉素诱导K562细胞凋亡的研究
Induction of apoptosis in K562 human chronic myelogenous leukemia cells by tetra-arsenic tetra-sulfide, STI571 and herbimycin
摘要
Abstract
Objective The therapeutic effect of chronic myeloid leukemia (CML) is undesirable. In order to find a new sensitive drug for CML, this study aims at exploring the effects of tetra-arsenic tetra-sulfide (As4S4) on human leukemia K562 cells. Methods The viability of K562 cells, represented by absorbance, was measured by MTS assay. The cells morphological changes were determined by Wright's staining and Hoechst33342 assay. The cell apoptosis was evaluated by DNA agarose gel electrophoresis and the cell apoptosis rate was measured by flow cytometry. Results The cell viability decreased significantly being cultured with 5.0 μmol/L As4S4, STI571 and herbimycin for 72 hrs (with absorbances of 0.32±0.04, 0.49±0.01 and 0.69±0.02, respectively). As4S4 and STI571 had more inhibition on the K562 cells viability than herbimycin (P<0.01), and there was no statistically significant difference between that of As4S4 and STI571. The effects of 1.0, 5.0 and 10.0 μmol/L As4S4 on K562 cells were time-dependent. When the concentration was lower than 1.0 μmol/L, As4S4 had little effect on K562 cells. After being cultured with 2.0 μmol/L As4S4 for 24 to 48 hrs, typical morphological changes of apoptosis appeared in the K562 cells. After being cultured with 5.0 μmol/L As4S4, STI571 and herbimycin for 72 hrs, the apoptosis rate of K562 cells were 68.8%, 56.7% and 35.5%, respectively. When the concentrations of As4S4 changed from 2.0 to 3.0 μmol/L, the apoptosis rate increased from 25.7% to 45.3%. There was no significant difference between the apoptosis rate of K562 cells induced by 5.0 and 10.0 μmol/L As4S4. Conclusions As4S4 with the concentration of 2.0 μmol/L could inhibit the growth of K562 cells efficiently through inducing apoptosis.关键词
四硫化四砷/STI571/除莠霉素/K562/凋亡Key words
Tetra-arsenic tetra-sulfide/STI-571/Herbimycin/K562/Apoptosis分类
医药卫生引用本文复制引用
叶启东,顾龙君,赵金彩,陈文高,张蓓,蒋黎敏,沈立松..四硫化四砷STI571和除莠霉素诱导K562细胞凋亡的研究[J].中国当代儿科杂志,2004,6(1):15-18,4.基金项目
Shanghai Science and Technology Foundation (No: 02DJ14011). （No: 02DJ14011）
