中国现代医学杂志2004，Vol.14Issue(19)：15-18,4.
叶酸、维生素B12,MTHFR基因及血浆同型半胱氨酸水平与Alzheimer病关系的研究
Relationship between folate,vitamin B12,plasma homocysteine levels and polymorphisms of MTHFR gene in Alzheimer's disease
摘要
Abstract
Objective:To study the relationship between plasma folate, vitamin B12, plasma homocysteine (Hcy) levels and the polymorphisms of MTHFR gene in Alzheimer's disease (AD). Methods: 42 AD patients and 40 normal aged subjects were recruited in the study. The polymorphisms of MTHFR gene were analyzed using PCR-RFLP and their plasma total Hcy levels were measured using high-performance liquid chromatography with electrochemical detection. Plasma folate and serum vitamin B12 were determined for all of them.Results: There were three kinds of genotype: T/T (homozygous mutation), T/C (heterozygous mutation) and C/C (wild-type). There was no significance between the frequencies of the genotypes and alleles of MTHFR gene in AD patients and in normal elderly subjects (P ＞0.05). Mean total plasma homocysteine concentrations were significantly higher in AD patients than in the normal elderly subjects (t=16.64, P＜0.001). Plasma folate and serum vitamin B12 concentrations were significantly lower in AD group than in control group (t=7.24, 9.11, re spectively, P ＜0.001). Conclusions: MTHFR mutation is not associated with AD. Hyperhomocysteinemia may be a new risk factor for AD.关键词
阿尔茨海默病/基因/半胱氨酸/叶酸/维生素B12Key words
Alzheimer's disease/gene/cysteine/folate/vitamin B12分类
医药卫生引用本文复制引用
毕胜,潘尚哈,张昱,吴江..叶酸、维生素B12,MTHFR基因及血浆同型半胱氨酸水平与Alzheimer病关系的研究[J].中国现代医学杂志,2004,14(19):15-18,4.基金项目
Supported by the postdoctoral foundation of China (No. LRB00071) （No. LRB00071）
