中国肺癌杂志2001，Vol.4Issue(1)：15-19,5.
野生型p14ARF基因转染对人肺癌细胞生长抑制的实验研究
Transfection of wild-type p14ARF gene leads to growth inhibition of human lung cancer cell lines
高楠 1胡义德 2黄抗美 1曹晓运 3周决 1曹世龙1
作者信息
- 1. 复旦大学医学院肿瘤研究所
- 2. 第三军医大学新桥医院全军呼吸内科研究所
- 3. 上海市华山医院神经外科
- 折叠
摘要
Abstract
Objective To examine whether wild-type p14ARF gene is a candidate suppressor gene for lung cancer. Methods Human lung cancer cell lines having various endogenous backgrounds in INK4a, p53 and Rb genes were used as the recipients of the wild-type p14ARF gene. The expression of p14ARF mRNA and protein was detected with RT-PCR, immunohistochemistry and Western blot after G418 selection. Clones which expressed both p14ARF mRNA and protein were identified and selected for further experiements. By comparing with the parental and negative control cells treated with empty vectors, the effects of exogenously transfected p14ARF on cell division rate, cell cycle distribution and morphologic alteration were analyzed. In vivo evaluation of the growth rate was also made with the experiment of nude mice tumor formation. Results Upon transfection with p14ARF gene, cells were arrested at G1 or G1／G2 phase of cell cycle in three wtp53 lung cancer cell lines and their proliferation rates were also inhibited. Conclusion Human wild-type p14ARF gene has suppressive effect on abnormal proliferation of lung cancer cells, especially in some wtp53 lung cancer cells, and it might be an ideal candidate for gene therapy of human lung cancer.关键词
p14ARF p53 肺肿瘤 细胞周期 抑癌基因分类
医药卫生引用本文复制引用
高楠,胡义德,黄抗美,曹晓运,周决,曹世龙..野生型p14ARF基因转染对人肺癌细胞生长抑制的实验研究[J].中国肺癌杂志,2001,4(1):15-19,5.
