中国现代医学杂志2004，Vol.14Issue(3)：15-19,22,6.
哮喘大鼠肺组织白血病抑制因子的表达及地塞米松干预研究
Expression of leukemia inhibitory factor in lung in ovalbumin sensitized and dexamethasone therapied rats
林敏娟 1胡成平 1潘频华 1吴鄂生1
作者信息
- 1. 中南大学湘雅医院,呼吸内科,湖南,长沙,410008
- 折叠
摘要
Abstract
Objective:To investigate the expression of leukemia inhibiObjective:Tory facObjective:Tor(LIF)in lung of ovalbumin sensitized and challenged rats for evaluating the role of LIF in pathogenesis of asthma.Methods:Asthma rat model sensitized and challenged by ovalbumin was established.The expression of LIF at mRNA and protein levels in the lung was detected by RT-PCR and western blotting.Results: Expression of LIF protein in the lungs of asthma rats(40832 ± 12964)was significantly elevated compared with control(23110 ± 8018,P＜0.01)and dexamethasone therapy(DXM)rats(16160 ± 2108,P＜0.01),but no difference was found between control group and DXM group(P ＞ 0.05).Expression of LIF mRNA in lungs of asthma rats(0.51 ± 0.13)was significantly elevated compared with control(0.24 ±0.02,P＜0.01)and DXM rats(0.18 ±0.05,P＜0.01),but no difference was found between control and DXM rats also(P ＞ 0.05).Conclusions: The expression of LIF at protein and mRNA levels are elevated significantly in ovalbumin sensitized rats,which indicates that LIF may play an important role in pathogenesis of asthma as a neuroimmune modulator.关键词
支气管哮喘/白血病抑制因子Key words
bronchial asthma/leukemia inhibitory factor分类
医药卫生引用本文复制引用
林敏娟,胡成平,潘频华,吴鄂生..哮喘大鼠肺组织白血病抑制因子的表达及地塞米松干预研究[J].中国现代医学杂志,2004,14(3):15-19,22,6.
