中华医学杂志（英文版）Issue(10)：1523-1529,7.
Effects of timely insulin treatment on protection of β cells in a rat model of type 2 diabetes mellitus
Effects of timely insulin treatment on protection of β cells in a rat model of type 2 diabetes mellitus
周迎生 1高妍 1郭晓蕙 1李斌 2王抒 3迟家敏4
作者信息
- 1. Department of Endocrinology,First Hospital of Peking University,Beijing 100034,China
- 2. Department of Plastic Surgery,Beijing Hospital,Beijing 100730,China
- 3. Department of Biochemistry,Beijing Institute of Geriatrics,Beijing 100730,China
- 4. Department of Endocrinology,Beijing Hospital,Beijing 100730,China
- 折叠
摘要
Abstract
Background Insulin treatment plays a key role in management of diabetes mellitus. Clinical researches showed that extra improvements in restoration of insulin secretion of pancreatic β cells were found in patients with newly diagnosed type 2 diabetes. The purpose of this study was to investigate the effects of early insulin treatment on insulin mRNA expression and morphological alterations of β cells in a Sprague Dawley (SD) rat model of type 2 diabetes. Methods A rat model of type 2 diabetes mellitus (T2DM) was induced by a high fat diet (high energy, HE) and low doses of streptozotoxin (STZ, 40 mg/kg). A group of diabetic rats was then injected with protamine zinc insulin (PZI, 1-2 U·kg-1·d-1) for one week. Insulin mRNA expression, morphological features of pancreatic islets, and metabolic parameters were examined in rats using reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and other techniques. Results In insulin-treated diabetic rats, insulin mRNA levels prominently increased by 81.3% (P<0.05), as compared with untreated diabetic rats. Moreover, timely insulin treatment noticeably improved the insulin content of β cells, with an increase of 10.2% (P<0.05), despite a slight reduction in fasting blood glucose (FBG), triglyceride (TG), and free fatty acid (FFA) levels, as compared to an untreated diabetic group. Conclusion Insulin treatment at the onset of T2DM effectively improves insulin synthesis, as confirmed by morphological changes to β cells in a rat model of type 2 diabetes.关键词
insulin/type 2 diabetes/rat model/β cellsKey words
insulin/type 2 diabetes/rat model/β cells分类
医药卫生引用本文复制引用
周迎生,高妍,郭晓蕙,李斌,王抒,迟家敏..Effects of timely insulin treatment on protection of β cells in a rat model of type 2 diabetes mellitus[J].中华医学杂志（英文版）,2004,(10):1523-1529,7.
