首页|期刊导航|中华医学杂志（英文版）|Role of pigment epithelium-derived factor on proliferation and migration of choroidal capillary endothelium induced by vascular endothelial growth factor in vitro
Role of pigment epithelium-derived factor on proliferation and migration of choroidal capillary endothelium induced by vascular endothelial growth factor in vitro
WANG Feng-hua SUN Xiao-dong ZHANG Xi XU Xun ZHU Qi HUANG Jian-nan FAN Ying GU Qing LIU Hai-yang
中华医学杂志（英文版）2007，Vol.120Issue(17)：1534-1538,5.
中华医学杂志（英文版）2007，Vol.120Issue(17)：1534-1538,5.
Role of pigment epithelium-derived factor on proliferation and migration of choroidal capillary endothelium induced by vascular endothelial growth factor in vitro
Role of pigment epithelium-derived factor on proliferation and migration of choroidal capillary endothelium induced by vascular endothelial growth factor in vitro
摘要
Abstract
Background Pigment epithelium-derived factor (PEDF) is expressed in several normal organs and identified as an inhibitor of neovascularization. In the present study, we investigated the effect of PEDF in an in vitro model of ocular choroidal neovascularization.Methods Microdissection was used to isolate the human choroidal endothelial cells (CECs), followed by the use of superparamagnetic beads (Dynabeads) coated with the CD31 antibody, which selectively binds to the endothelial cell surface. The mitogenic and motogenic effects of vascular endothelial growth factor (VEGF) on cultured choroidal capillary endothelial cells were examined in the presence or absence of PEDF (1, 10, 100, and 1000 ng/ml) using cell counts and migration assays.Results Cells bound to the beads were isolated using a magnetic particle concentrator and they were successfully cultured and characterized to be endothelial cells that possessed greater than 95% immunoreactivity to von Willebrand factor. PEDF suppressed the proliferation and migration of VEGF-induced choroidal capillary endothelial cells. However,the concentration of PEDF which we used has little effect on normal CECs.Conclusions PEDF played an important role on the growth and migration of VEGF-stimulated choroidal endothelial cell.These findings suggest that PEDF may be an effective approach to the treatment of choroidal neovascular disorders.关键词
choroidal neovascularization/ cell cultivation/ pigment epithelium-derived factorsKey words
choroidal neovascularization/ cell cultivation/ pigment epithelium-derived factors分类
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WANG Feng-hua,SUN Xiao-dong,ZHANG Xi,XU Xun,ZHU Qi,HUANG Jian-nan,FAN Ying,GU Qing ,LIU Hai-yang..Role of pigment epithelium-derived factor on proliferation and migration of choroidal capillary endothelium induced by vascular endothelial growth factor in vitro[J].中华医学杂志（英文版）,2007,120(17):1534-1538,5.基金项目
This study was supported by grants from Shanghai Science and Technology Committee "Qi Ming Xing" Project (No. 02QB 14037) and Youth Foundation of Shanghai Jiao Tong University, School of Medicine (No. 040202). （No. 02QB 14037）
