中德临床肿瘤学杂志（英文版）2008，Vol.7Issue(3)：154-158,5.
鞘内注射P38MAPK抑制剂对乳腺癌骨转移大鼠疼痛行为及前炎性细胞因子表达的影响
Effects of p38 MAPK inhibitor on the rat pain behavior and proinflammatory cytokines in a metastatic bone cancer pain model
摘要
Abstract
Objective: To observe the effects of p38 mitogen activated protein kinase (MAPK) inhibitor SB203580 by intrathecal injection on the pain behavior and the spinal proinflammatory cytokines in a rat model of bone cancer pain induced by breast cancer cells. Methods: Eleven rats were used to establish the models of bone cancer pain, six rats were treated by intrathecal SB203580 injection, and the other 5 were as the controls. The paw withdrawal latency (PWL), histology and the spinal levels of IL-1β and TNF-α were detected. Results: All the 11 rats presented evident bone destruction and thermal hyperalgesia after intra-tibial injection of breast cancer cells. No effect of SB203580 on the bone destruction was observed.However, following intrathecal injection of SB203580, the left PWLs (12.12±1.26 s at 16 days and 12.99±1.65 s at 19 days)were significant higher than that of controls (9.05±1.08 s at 16 days and 8.55±1.60 s at 19 days), P＜0.05. Meanwhile,intrathecal injection of SB203580 evidently reduced the levels of spinal IL-1βand TNF-α. Conclusion: Intrathecal injection of SB203580 in a rat model of bone cancer pain cannot prevent the tibial destruction but significantly depress the thermalgia sensitivity, which might result from inhibiting intracellular p38 MAPK signaling transduction, and thereby reducing the release of the proinflammatory cytokines.关键词
p38 MAPK inhibitor/bone cancer pain/ thermal hyperalgesia/ proinflammatory cytokineKey words
p38 MAPK inhibitor/bone cancer pain/ thermal hyperalgesia/ proinflammatory cytokine分类
医药卫生引用本文复制引用
Cuiju Tang,Shiying Yu,Min Zhang,Rui Jiang,Na Li ,Huiting Xu..鞘内注射P38MAPK抑制剂对乳腺癌骨转移大鼠疼痛行为及前炎性细胞因子表达的影响[J].中德临床肿瘤学杂志（英文版）,2008,7(3):154-158,5.基金项目
Supported by a grant from the National Nature Sciences Foundation of China (No. 30672426). （No. 30672426）
