热带亚热带植物学报2005，Vol.13Issue(2)：154-158,5.
人工合成的植物化猪α乳清蛋白基因在转基因拟兰芥的表达
Expression of Synthetic Porcine Alpha-Lactalbumin in Transgenic Arabidopsis
摘要
Abstract
A 398-bp plantified encoding fragment of porcine α -lactalbumin, was cloned into plasmid under the control of the cauliflower mosaic virus 35S promoter. The expression cassette was introduced into A rabidopsis with Agrobacterium mediated transformation. Some herbicide-resistant lines of transformed A rabidopsis were selected. The gene integration of chromosomal DNA level were confirmed in regenerated plants. All of them are positive for porcine α-lactalbumin gene via genomic DNA PCR. The expression at the protein level could be confirmed only in the transgenic Arabidopsis. The arabidopsis-expressed lactalbumin (LA) migrated in SDSPAGE with identical mobility to human LA prepared from human milk, indicating that the LA was correctly processed to yield a mature protein in plants关键词
猪α乳清蛋白/拟兰芥/转化/蛋白质表达Key words
Porcine α-lactalbumin/A rab idopsis/Transformation/Expression分类
农业科技引用本文复制引用
芦春斌..人工合成的植物化猪α乳清蛋白基因在转基因拟兰芥的表达[J].热带亚热带植物学报,2005,13(2):154-158,5.基金项目
Supported by United Soybean Board Domestic Program Project in USA （）
