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Establishment and Application of a Set of Material System for Studying the Resistance to Blast in Rice

LI Xiao-fang LUO Wen-yong XIAO Xin MAO Xing-xue LIU Yan-zhuo

中国农业科学(英文版)2003,Vol.2Issue(2):154-162,9.
中国农业科学(英文版)2003,Vol.2Issue(2):154-162,9.

Establishment and Application of a Set of Material System for Studying the Resistance to Blast in Rice

Establishment and Application of a Set of Material System for Studying the Resistance to Blast in Rice

LI Xiao-fang 1LUO Wen-yong 2XIAO Xin 1MAO Xing-xue 1LIU Yan-zhuo1

作者信息

  • 1. Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P. R. China
  • 2. Hubei Agricultural College, J ingzhou 434103, P. R. China
  • 折叠

摘要

Abstract

The study used Waixuan35, a blast resistant rice material adopted widely previously in South China, Yuexiangzhan, a rice variety with the largest planting area in Guangdong Province at present and with the characteristics of horizontal blast resistance and high harvest index, and Qisizhan, the earliest good eating quality variety in Guangdong Province as parent materials, and created an important material systems for studying agricultural characters such as rice blast resistance genes, their resistance mechanisms and quality by hybridization offspring single grain transfer method and DNA hybridization method, namely recombination inbred line RIL, extend recombination inbred line ERIL and transferred-mutant recombination inbred line TRIL assemblages. In RIL assemblage, various materials of resistance gene combinations for identifying rice blast pathogen strains had been identified from the material system, providing direct material evidence for gene to gene theory. Using the same control and same method, in ERIL assemblage, it was proved that there were several main resistance genes and several minor resistance genes in horizontal resistant Yuexiangzhan,providing preliminary evidences for multigene control in horizontal resistance. A set of stable material assemblage for studying future horizontal resistance had been created. A mutation material of Yuexiangzhan from horizontal resistance to vertical resistance was obtained by wild rice external DNA electric stimulation introduction, and variation in mutation assemblage was certified by SSLP. These studies and materials provided important new materials for studying rice blast resistance mechanisms, gene cloning, resistance genes especially horizontal resistance.

关键词

Rice/Blast resistance/RIL assemblage

Key words

Rice/Blast resistance/RIL assemblage

分类

农业科技

引用本文复制引用

LI Xiao-fang,LUO Wen-yong,XIAO Xin ,MAO Xing-xue,LIU Yan-zhuo..Establishment and Application of a Set of Material System for Studying the Resistance to Blast in Rice[J].中国农业科学(英文版),2003,2(2):154-162,9.

基金项目

The research is supported by Important and Key Project of the National Natural Science Foundation of China(39893350 and 30040025) and Key Project of Natural Science Fund of Guangdong Province(020384). (39893350 and 30040025)

中国农业科学(英文版)

2095-3119

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