南京医科大学学报（英文版）2006，Vol.20Issue(3)：155-159,5.
Effects of arsenic trioxide on drug transporting molecules in multidrug resistance malignant neoplasma MR2 cell line
Effects of arsenic trioxide on drug transporting molecules in multidrug resistance malignant neoplasma MR2 cell line
Xiaoping Qian 1Baorui Liu 1Yang Yang 1Lifeng Wang 1Zhengyun Zou 1Weiwei Kong 1Juan Du1
作者信息
- 1. Department of Oncology, Nanjing Drum Tower Hospital, Nanjing traditional Chinese medicine University, Nanjing 210008, China
- 折叠
摘要
Abstract
Objective: To study the effect of arsenic trioxide (As2O3) on the expression of drug transporting molecules in multidrug resistance malignant neoplasma acute promyelocytic leukemia (APL) MR2 cell line. Methods: MR2 resistant to alltrans retinoic acid (ATRA) and non-ATRA resistant APL cell line NB4 were used. Expressions of P-glycoprotein (Pgp), multidrug resistance protein (MRP) and lung resistance-related protein (LRP) were detected by immunocytochemical assay. Results:The expression of Pgp was significantly higher in MR2(30%-40%) than that in NB4 (10%-20%) (P ＜ 0.001), and the expression of MRP was also higher in MR2 (56.9 ± 3.4 ～ 21.2 ± 1.1) than that in NB4 (20.6 ± 5.3 ～ 16.7 ± 1.2) (P ＜ 0.001). As2O3 ranging from 0.5-2.0 μmol/L could significantly decrease the expressions of Pgp and MRP. The expression of Pgp and MRP in MR2 cell line were negatively correlated with the dose and duration of action of As2O3. Conclusion: Pgp and MRP may be the sensitive targets of As2O3 to overcome drug-resistance. ATRA might be the substrates of Pgp and MRP.关键词
arsenic trioxide/neoplasma, P-glycoprotein, multidrug resistance protein, lung resistancerelated proteinKey words
arsenic trioxide/neoplasma, P-glycoprotein, multidrug resistance protein, lung resistancerelated protein分类
医药卫生引用本文复制引用
Xiaoping Qian,Baorui Liu,Yang Yang,Lifeng Wang,Zhengyun Zou,Weiwei Kong,Juan Du..Effects of arsenic trioxide on drug transporting molecules in multidrug resistance malignant neoplasma MR2 cell line[J].南京医科大学学报（英文版）,2006,20(3):155-159,5.
