中华医学杂志（英文版）Issue(10)：1552-1557,6.
Electron paramagnetic resonance in monitoring of nitric oxide production after kidney transplantation in rats
Electron paramagnetic resonance in monitoring of nitric oxide production after kidney transplantation in rats
徐涛 1陈希 2王晓峰 1黄晓波 1曲星珂 1叶海云 1张小东 1侯树坤 1朱积川1
作者信息
- 1. Department of Urology,Peking University People's Hospital,Beijing 100044,China
- 2. Department of Cell Biology and Genetics,Peking University Health Science Center,Beijing 100083,China
- 折叠
摘要
Abstract
Background Much research has been focused on ischemia/reperfusion injury (IRI) to the transplanted organs. As a free radical, nitric oxide (NO) plays an important role in IRI. In this study, the production of NO and its functions during IRI were monitored in rat models after allotransplantation of kidney grafts.Methods Of 75 male LEW rats, 30 served as donors, and the remaining 45 rats were divided into three groups (15 rats in each group): controls (group 1), kidney allotransplantation followed by bilateral nephrectomy during reperfusion (group 2), 2 hours before operation, donors and recipients were treated with NG-nitro L-arginine methyl ester (L-NAME), a NO synthase inhibitor, at a dose of 30 mg/kg (group 3). Bilateral nephrectomies were performed while kidney grafts were reperfused. The kidney grafts were hypothemically stored for 24 hours. The production of NO before and after reperfusion was measured by electron paramagnetic resonance (EPR). The creatinine level, the glomerular filtration rate (GFR) and the protein carbonyl content in tissue samples were recorded on the first and the fifth day after operation. The data were evaluated by one-way analysis of variance. Differences were considered to be statistically significant when a P value was less than 0.05.Results After reperfusion for 15 minutes, the production of NO increased remarkably and kept increasing till 120 minutes, after which the level returned to normal. In group 3, which was pretreated with L-NAME, creatinine levels were higher than those in group 2 at the 24th hour (4.10±0.50 mg/dl vs. 3.77±0.42 mg/dl, P<0.05) and the 120th hour (3.19±0.79 mg/dl vs. 2.22±0.53 mg/dl, P<0.05). GFR levels in group 3 were lower than those in group 2 at the 24th hour (0.50±0.12 ml/min vs. 0.71±0.19 ml/min, P<0.05) and the 120th hour (0.59±0.38 ml/min vs. 1.27±0.23 ml/min, P<0.01). The content of protein carbonyl in tissue samples of group 3 was lower than that in group 2 at the 24th hour (29.01±7.02 nmol/mg protein vs. 49.39±13.13 nmol/mg protein, P<0.05), but was higher than that at the 120th hour (75.71±16.74 nmol/mg protein vs. 57.93±15.32 nmol/mg protein, P<0.05).Conclusions After transplantation of hypothemically stored kidney grafts, the increased NO production in the early stage has protective effects on the transplanted kidney. Application of L-NAME to inhibit NO production is harmful to the recovery of the renal functions of kidney grafts.关键词
nitric oxide/kidney transplantation/ischemia/reperfusion injury/electron paramagnetic resonanceKey words
nitric oxide/kidney transplantation/ischemia/reperfusion injury/electron paramagnetic resonance分类
医药卫生引用本文复制引用
徐涛,陈希,王晓峰,黄晓波,曲星珂,叶海云,张小东,侯树坤,朱积川..Electron paramagnetic resonance in monitoring of nitric oxide production after kidney transplantation in rats[J].中华医学杂志（英文版）,2004,(10):1552-1557,6.
