物理化学学报2008，Vol.24Issue(9)：1558-1562,5.
含有D型氨基酸的新型毒肽——芋螺马芬在pH 5的溶液结构
Solution Structure of Conomarphin, a Novel Conopeptide Containing D-Amino Acid at pH 5
摘要
Abstract
Conomarphin, a novel conopeptide containing D-amino acid, was identified from the venom of Conus marmoreus and classified into M-superfamily of conotoxin. In this article, we reported the 3D structure of conomarphin at pH 5 determined using 2D 1H NMR method in aqueous solution. Twenty converged structures of this peptide were obtained based on 205 distance constraints, 8 dihedral angle constraints, and 2 hydrogen bond constraints. The root mean square deviation (RMSD) values of the backbone atoms were (0.074±0.029) nm. The refined structure of conomarphin at pH 5 contained a short 310-helix at C-terminal of the peptide. It was also characterized by a loose loop centered at Ala6. Comparison of structural and electrostatic potential between conomarphin at pH 3 and pH 5 were presented. Although the solution structure of conomarphin at pH 5 shared part of the same secondary structure element with the structure of conomarphin at pH 3, it adopted a distinctive backbone conformation with the overall molecule resembling a "flexcual arm" when viewed from the front. Structural differences imply that this conopeptide is rather pH sensitive and its bioactivity in vivo might be related to the acidity.关键词
芋螺马芬/芋螺肽/D-苯丙氨酸/NMR溶液结构/pH敏感Key words
Conomarphin/Conopeptide/D-Phenylalanine/NMR solution structure/pH sensitive分类
化学化工引用本文复制引用
黄飞娟,杜为红,王保怀..含有D型氨基酸的新型毒肽——芋螺马芬在pH 5的溶液结构[J].物理化学学报,2008,24(9):1558-1562,5.基金项目
国家自然科学基金(20473013,20633080)、教育部科技重点项目(108121)及国家基础研究项目(2004CB719900)资助 （20473013,20633080）
