生态学报2007，Vol.27Issue(4)：1558-1564,7.
海南椰心叶甲病原菌金龟子绿僵菌的分离、鉴定及其生防潜力
Isolation and identification of Metarhizium anisopliae from natural infections of Coconut hispid beetle Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae) , and preliminary studies on against this pest in Hainan island, China
摘要
Abstract
Coconut hispid beetles, Brontispa longissima (Gestro) (Coleoptera:Chrysomelidae) have been prevalent all over Hainan Island in China, and millions of coconut palms were attacked by this pest. The objective of this work was to isolate and identify strains of entomopathogenic fungi from natural infections of B. longissima collected from coconut palms.Conidia or mycelia collected from the surface of beetle cadavers were cultured and the colonies were screened on dodine oatmeal agar medium (DOA). The colony morphology, mycelia growth rate and conidial production were further studied on various agar media: PPDA, OMA, V8A and PDA. In order to confirm whether these isolates were pathogenic to B.longissima, their virulence to the beetles were also tested in laboratory. The results showed two different kinds of strains MA and MB were all identified as Metarhizium anisopliae(Metschnikoff) base on the macromorphological and micromorphological characteristics, and PPDA was the optimal culture medium for vegetative growth and conidial production. Several high virulent strains were screened in vitro, and application on controlling coconut hispid beetles was carried out preliminarily in vivo. The results showed a significant reduction in adult population was caused by the isolate. It was proved that M.anisopliae isolated from the natural infected cadavers of B. longissima have great potential as a means of biocontrol against this serious pest.关键词
金龟子绿僵菌/分离/鉴定/生物防治/椰心叶甲Key words
Metarhizium anisopliae/isolation/identification/biological control/Brontispa longissima分类
生物科学引用本文复制引用
詹儒林,覃伟权,宋妍,张世清,H H HO,许天委,黄俊生..海南椰心叶甲病原菌金龟子绿僵菌的分离、鉴定及其生防潜力[J].生态学报,2007,27(4):1558-1564,7.基金项目
国家科技基础条件平台建设资助项目(2005DKA21201-18) （2005DKA21201-18）
热带作物生物技术国家重点实验室开放基金资助项目 The project was financially supported by National Science and Technology Foundation Platform Construction Item( No.2005DKA21201-18) （ No.2005DKA21201-18）
Opening Foundation of State Key Laboratory of Tropical Crop Biotechnology （）
