中华医学杂志（英文版）2008，Vol.121Issue(16)：1563-1568,6.
Low expression of SLOOP associated with paclitaxel resistance in ovarian cancer cell line
Low expression of SLOOP associated with paclitaxel resistance in ovarian cancer cell line
GAO Jian-hua 1HE Zhi-juan 1WANG Qi 1LI Xin 1LI Yi-xuan 2LIU Min 2ZHENG Jian-hua 1TANG Hua2
作者信息
- 1. Department of Obstetrics and Gynecology, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150010, China
- 2. Tianjin Life Science Research Center, Tianjin Medical University, Tianjin 300070, China
- 折叠
摘要
Abstract
Background Recent studies indicate that Sl 00P expression may be a biomarker that can predict the success of cancer chemotherapy. Whether it is relevant to chemOtherapeutics in ovarian cancer is unknown.In this study,we investigated the association of S1OOP expression with paclitaxel sensitivity in ovarian cancer cell lines.Methods We measured S1 OOP expression and paclitaxel resistance profiles in parent SKOV3 and OVCAH3 cell lines.Then,the two cell lines were transiently transfected with SlOOP siRNA.We also constructed an OVCAR3 cell clone that stably overexpressed SIOOP The effect of S100P expression level on the survival of cells exposed to paclitaxel was measured using the MTT assav.S1OOP expression was evaluated by semi-quantitative RT.PCR and Western blotting.Significance of differences was calculated using independent samples t-test and one way analysis of variance(ANOVA).Results Lower S1 00P expression was associated with a survival advantage in OVCAR3 cells exposed to paclitaxel;the survival advantage in SKOV3 cells was smaller P<O.05).The survival advantage associated with decreased S1 OOP expression was even greater for SKOV3 and OVCAR3 cells that had been transfected with S1 00P siRNA before being exposed to paciitaxel(P<0.05).Consistent with this,the OVCAR3 cell clone that was transfected to overexpress S1 00P was more sensitive to paclitaxelf P<0.05).Conclusions Low S1 00P expression contributes to drug resistance to paclitaxel in ovarian cancer cell lines.S100P expression thus might be a marker that can predict the effectiveness of paclitaxel based chemotherapy.Such a marker could be helpful in improving individual medication regimens for ovarian cancer patients.关键词
drug resistance/paclitaxel/ovarian cancer/calcium-binding proteinsKey words
drug resistance/paclitaxel/ovarian cancer/calcium-binding proteins分类
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GAO Jian-hua,HE Zhi-juan,WANG Qi,LI Xin,LI Yi-xuan,LIU Min,ZHENG Jian-hua,TANG Hua..Low expression of SLOOP associated with paclitaxel resistance in ovarian cancer cell line[J].中华医学杂志（英文版）,2008,121(16):1563-1568,6.
