中国临床康复2004，Vol.8Issue(8)：1566-1567,2.
同种异体神经修复鼠坐骨神经缺损的实验研究
An experimental study on allogenic nerve grafts repairing the gap of rat sciatic nerve
刘世清 1张思胜 1彭昊 1陶海鹰 1范里1
作者信息
- 1. 武汉大学人民医院骨科,湖北省武汉市,430060
- 折叠
摘要
Abstract
AIM:To study the effect of nerve regeneration through bridging the gap of rat sciatic nerve with acellular basal laminea tubes. METHODS:Acellular basal lamina tubes were acquired from normal rat sciatic nerves which were treated with nondenaturing biological detergents.Thirty adult SD rats were divided randomly into three groups, with 10 in each group. Acellular basal lamina tubes graft group A);autogenous nerve bridging(group B); allogenic nerve bridging(group C). A gap of 20 mm of rat left sciatic nerve was extracted, which was bridged by different nerve materials.Histomorphometry, electromyographic and image analysis were determined at 2 and 3 months after surgical operation. RESULTS:A number of axons in regenerating nerve were found to cross the graft, the latency and wave amplitude of regenerating nerve in group A were lower than that in group B(ta=2.101,P< 0.05;tb=5.00, P< 0.05)at 2 months, there was no statistical difference betwen the two groups at 3 months.The thickness of myelin sheath of experimental group was inferior to group B(ta=5.68,P< 0.05)at 3 months, while the number and diameter of axons in both groups were similar. CONCLUSION: The graft is found to support outgrowth of axons and migration of Schwann cell,and is a good tool to use in peripheral nerve regeneration. 关键词
坐骨神经/神经移植/生物相容性分类
医药卫生引用本文复制引用
刘世清,张思胜,彭昊,陶海鹰,范里..同种异体神经修复鼠坐骨神经缺损的实验研究[J].中国临床康复,2004,8(8):1566-1567,2.
