中华医学杂志（英文版）2009，Vol.122Issue(13)：1570-1574,5.DOI:10.3760/cma.j.issn.0366-6999.2009.13.018
Low molecular weight heparin suppresses lymphatic endothelial cell proliferation induced by vascular endothelial growth factor C in vitro
Low molecular weight heparin suppresses lymphatic endothelial cell proliferation induced by vascular endothelial growth factor C in vitro
CAO Guang 1WU Ji-xiang 2WU Qing-hua2
作者信息
- 1. Department of General Surgery ,Anzhen Hospital,Capital Medical University,Beijing 100029,China
- 2. Department of Vascular Surgery ,Anzhen Hospital,Capital Medical University,Beijing 100029,China
- 折叠
摘要
Abstract
Background Pancreatic cancer is one of the most aggressive human malignancies. Lymphangiogenesis plays an important role in lymph node metastasis of many solid tumors. It is well known that low molecular weight heparins (LMWHs) can inhibit cell growth, cell invasion and angiogenesis, which are key processes in tumor progression. Methods We measured the expression of vascular endothelial growth factor C (VEGF-C) in pancreatic cancer cells (PANC-1) using reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. We used an in vitroassay to evaluate the anti-lymphangiogenic effect of an LMWH, Fragmin, on human lymphatic endothelial cell (HLEC) proliferation. Results Fragmin at a low concentration can effectively inhibits HLEC proliferation induced by VEGF-C. VEGF-C secreted by PANC-1 cells stimulated HLEC proliferation. Low concentration LMWH suppressed HLEC proliferation induced by VEGF-C but did not affect proliferation or VEGF-C expression of PANC-1 cells, whereas high concentrations of LMWH inhibited PANC-1 cell proliferation. Conclusions These results suggest that VEGF-C released by cancer cells plays an important role in promoting HLEC proliferation. The LMWH Fragmin has anti-lymphangiogenic effects and may inhibit lymphatic metastasis in pancreatic cancer.关键词
low molecular weight heparin/ vascular endothelial growth factor C/ human lymphatic endothelial cell/ pancreatic cancer cell/ proliferationKey words
low molecular weight heparin/ vascular endothelial growth factor C/ human lymphatic endothelial cell/ pancreatic cancer cell/ proliferation分类
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CAO Guang,WU Ji-xiang,WU Qing-hua..Low molecular weight heparin suppresses lymphatic endothelial cell proliferation induced by vascular endothelial growth factor C in vitro[J].中华医学杂志（英文版）,2009,122(13):1570-1574,5.
