首页|期刊导航|中国农业科学（英文版）|Photosynthetic Characteristics and Antioxidative Metabolism of Flag Leaves in Responses to Nitrogen Application During Grain Filling of Field-Grown Wheat
Photosynthetic Characteristics and Antioxidative Metabolism of Flag Leaves in Responses to Nitrogen Application During Grain Filling of Field-Grown Wheat
CAI Rui-guo WANG Zhen-lin ZHANG Min YIN Yan-ping WANG Ping ZHANG Ti-bin GU Feng DAI Zhong-min LIANG Tai-bo WU Yun-hai
中国农业科学（英文版）2008，Vol.7Issue(2)：157-167,11.
中国农业科学（英文版）2008，Vol.7Issue(2)：157-167,11.
Photosynthetic Characteristics and Antioxidative Metabolism of Flag Leaves in Responses to Nitrogen Application During Grain Filling of Field-Grown Wheat
Photosynthetic Characteristics and Antioxidative Metabolism of Flag Leaves in Responses to Nitrogen Application During Grain Filling of Field-Grown Wheat
摘要
Abstract
A two-factorial experiment was conducted with two wheat cultivars,SN1391(large spike and large grain)and GC8901(multiple spike and medium grain),and two nitrogen(N)application rates(12 and 24 g N m-2),to investigate the responses of photosynthetic characteristics and antioxidative metabolism to nitrogen rates in flag leaves of field-grown wheat during grain filling.The results showed that the content of N and chlorophyll(Chl)in wheat flag leaves decreased after anthesis and the net photosynthetic rate(Pn),effective quantum yield of PS II(ΦPS Ⅱ),efficiency of excitation capture by open PS H reaction centers(Fv'/Fm'),and photochemical quenching coefficient(qp)began to decrease at 14 days after anthesis.However,the maximal efficiency of PS II photochemistry(Fv/Fm)decreased slightly until the late period of senescence and the nonphotochemical quenching coefficient(NPQ)increased during flag leaves' senescence.As a result,a conflict came into being between absorption and utilization to light energy in flag leaves during senescence,which might accelerate the senescence of flag leaves.Compared with GC8901,the lower plant population of SN1391during grain filling was helpful to maintain the higher content of photosynthetic pigment,activity of PS U,and Pn in flag leaves during senescence.The delayed decrease in antioxidative enzyme activity and the lower degree of membrane lipid peroxidation in the senescing leaves of SN1391 were beneficial to protect the photosynthetic apparatus,which lead to the prolonged duration of CO2 assimilation.With the increase of N application,the Chl content of SN1391 flag leaves and the efficiency of excitation captured by open PS II centers increased.At the same time,the thermal dissipation in SN1391 flag leaves at high N(HN)treatment decreased and ?PsⅡimproved greatly,which were favorable to the increase of Pn.The SOD,POD,CAT and APX activities in the flag leaves of SN1391 increased markedly at HN treatment,indicating that these enzymes could clean more active oxygen and decrease the degree of membrane lipid peroxidation.In this way,the ability of SN1391 to protect photosynthetic apparatus was improved with the increase of N.In the HN treatment,the decreased activity of PS II and increased thermal dissipation resulted in the decline of Pn in flag leaves of GC8901.Meanwhile,the decreased antioxidative enzyme activities and the increased degree of membrane lipid peroxidation had indirect and unfavorable influences on CO2 assimilation.This implied that the conflict between absorption and utilization to energy in senescing leaves was an important reason which induced and accelerated the senescence of wheat leaves in the field.The photosynthetic characteristics and antioxidative metabolism of flag leaves during grain filling were markedly different among wheat cultivars.The effects of nitrogen rate on the photosynthetic and senescent characteristics of flag leaves also varied with wheat cultivars.关键词
wheat(Triticum aestivum L.),photosynthetic characteristics,antioxidative metabolism,nitrogenKey words
wheat(Triticum aestivum L.),photosynthetic characteristics,antioxidative metabolism,nitrogen分类
农业科技引用本文复制引用
CAI Rui-guo,WANG Zhen-lin,ZHANG Min,YIN Yan-ping,WANG Ping,ZHANG Ti-bin,GU Feng,DAI Zhong-min,LIANG Tai-bo,WU Yun-hai..Photosynthetic Characteristics and Antioxidative Metabolism of Flag Leaves in Responses to Nitrogen Application During Grain Filling of Field-Grown Wheat[J].中国农业科学（英文版）,2008,7(2):157-167,11.基金项目
The authors are grateful to the support by the National Natural Science Foundation of China (30270781,30571099). （30270781,30571099）
