中华医学杂志（英文版）2002，Vol.115Issue(10)：1579-1581,3.
I-Ag7和I-Ad四聚体的交叉反应性
The cross-reactivity of I-Ag7 and I-Ad tetramers
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作者信息
- 1. 湖南医科大学附三院神经内科,湖南,410013;Division of Immunology, Beckman Research Institute, City of Hope National Medical Center, CA 91010, USA
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摘要
Abstract
Objective To investigate the cross-reactivity between glutamic acid decarboxylase (GAD)- I-Ag7 and I-Ad tetramer in diabetes-prone non-obese diabetic (NOD) mice (I-Ag7) and diabetes-free Balb/c mice (I-Ad). Methods Two GAD peptide I-Ag7 and I-Ad tetramers were generated and compared for phenotype and function of sorted GAD peptide I-Ag7 and I-Ad tetrame r-positive (tet+) T cells.Results The cross-reactivity is shown in either tetramer positive percentage or tetramer staining intensity. The NOD and Balb/c derived-tet+ T cells were able to be cross-stained by GAD peptide I-Ag7 and I-Ad tetramers, and responded to both irradiated NOD and Balb/c splenotyes under stimulation by synthetic an drecombinant GAD peptides. Conclusion Although I-Ag7 and I-Ad are closely related in biochemical and biological aspects, their most notable difference is the presence or absence of a negat ively charged residue at position β57 that links to insulin-dependent diabetes mellitus.关键词
抗原肽-MHC四聚体 谷氨酸去羰基酶 I型糖尿病 交叉反应性Key words
peptide-MHC tetramer/glutamic acid decarboxyl ase/diabetes mellitus, insulin-dependent/cross-eactivity分类
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..I-Ag7和I-Ad四聚体的交叉反应性[J].中华医学杂志（英文版）,2002,115(10):1579-1581,3.
