中国临床康复2004，Vol.8Issue(8)：1580-1581,2.
染料木黄酮对前列腺癌细胞系PC-3生物学行为影响的意义
Effect of genistein on the biological behavior of PC-3 cell line of prostatic carcinoma
高晓康 1杨波 1王禾 1刘贺亮 1邵晨 1邵国兴 1康福霞1
作者信息
- 1. 解放军第四军医大学西京医院泌尿外科,陕西省西安市,710033
- 折叠
摘要
Abstract
AIM:To investigate the effect of genistein on the biological behavior of PC-3 cell line of carcinoma of prostate and explore the possibility of genistein used as a preventive drug. METHODS:PC-3 cells were exposed to conditional media containing 0, 10,20, 40 μ mol/L genistein for three days . The proliferation of PC-3 cells was studied by MTT analysis.The effects of genistein on cell cycle were detected by FCM. We also investigated the abilities of the genistein-treated cells to invade through the basement membrane incursive model reconstructed by using Transwell chambers. RESULTS:The proliferation was inhibited in a time-dependent and a dose-dependent manner after treating with genistein,and the IC50 of genisein was 25 μ mol/L after 3 days treatment. Cell cycle was blocked at G2/M stage,the proportion of G1 stage decreased in a time-dependent and a dose-dependent manner,and the percent of G2/M cells was 14.9%, 27.4% ,33.1% ,31.9% in the 0,10,20 and 40 μ mol/L genistein group respectively.The percent of apoptosis cells was 0%, 6.5% ,14.2% ,25.4% respectively. The ability of genistein-treated cells to invade the reconstituted basement membrane decreased to 31.8%, 8.6% and 3.96% in comparison with that of the control group. CONCLUSION:Genitein inhbits the invasion of PC-3 cells in a dose-dependent manner. Genistein may act as a protective drug for prostatic cancer by inhibiting the proliferation,inducing apoptosis and decreasing the invasive ability of PC-3 cells. 关键词
前列腺肿瘤 /预防和控制/癌/酶抑制剂分类
医药卫生引用本文复制引用
高晓康,杨波,王禾,刘贺亮,邵晨,邵国兴,康福霞..染料木黄酮对前列腺癌细胞系PC-3生物学行为影响的意义[J].中国临床康复,2004,8(8):1580-1581,2.
