中国药理学通报2001，Vol.17Issue(2)：158-160,3.
血管紧张素Ⅱ介导心肌成纤维细胞增殖的信号转导
Signaling transconduction mediated cultured rat cardiac fibroblasts proliferation by angoitensin Ⅱ
杨永健 1周兴文 1张鑫 1朱峻 1赵龙生1
作者信息
摘要
Abstract
AIM To study the effect of calcineurin (CaN)-dependent signaling pass way on proliferation of rat cardiac fibroblasts (FBs) under stimulation of angoi tensin Ⅱ (Ang Ⅱ). METHODS Upon the model of cultured rat FBs, C a2+ influx stimulated with Ang Ⅱ,CaN signaling pathway was blocked with c yclosporine A (CsA )and Ca2+ channel with verapamil(Ver), detecting the ac tivities of FBs CaN, mitogen activated protein kinase （MAPK） and protein kinas e C(PKC), ［3H］-leucine(［3H］-Leu) and ［3H］-thymidine( ［3H］-Td R) incorporation as the target to evaluate FBs proliferation. RESULTS Synthesis rates of protein and nucleic acid stimulated by Ang Ⅱ in FBs in creased significantly in contrast to control (P<0.01);CsA and Ver markedly inhibited syntheses of protein and nucleic acid mediated by Ang Ⅱ in FBs with a significant difference from Ang Ⅱ-stimulated group (P<0.01).CsA and Ver also suppressed FBs CaN activity mediated by Ang Ⅱ, and Ver suppressed FBs PKC activity mediated by Ang Ⅱ. CONCLUSION The study indicates CaN signaling pathway play an important role in FBs proliferation induced by Ang Ⅱ, but it is not the single signaling pathway in regulating FBs proliferation, MA PK-centered signaling pathway is also involved in FBs proliferation induced by Ang Ⅱ.关键词
钙调神经磷酸酶/成纤维细胞,心脏/细胞增殖/信号通路/血管紧张素Ⅱ/环孢素A/维拉帕米分类
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杨永健,周兴文,张鑫,朱峻,赵龙生..血管紧张素Ⅱ介导心肌成纤维细胞增殖的信号转导[J].中国药理学通报,2001,17(2):158-160,3.
