中国医学科学院学报2001，Vol.23Issue(2)：158-162,5.
小鼠胸腺增龄相关性基因的差异表达及其克隆
Differential Expression Analysis and Cloning of Murine Thymic Aged-related Genes
李永海 1苏华 1何维 1崔莲仙 1越智宏伦1
作者信息
- 1. 中国医学科学院中国协和医科大学基础医学研究所
- 折叠
摘要
Abstract
Objective Differential expression analysis and cloning of murine thymic aged-related genes.
Methods Different expressions of thymic mRNAs from 1- and 10-month old mice were analyzed via
DDRT-PCR and different expression sequence tags (ESTs) were obtained, following by identification with Northern Blotting, DNA sequencing, as well as screening of cDNA library. Results It was found that there would be a significant difference of gene expression in murine thymuses. Gene expression of some genes were exclusive in thymus from 1 or 10-month old mice, while some expressed different with age.108 differential display cDNA fragments were recovered, among which 31 were positive for hybridization by Northern blot. After sequenced, 14 ESTs were found to share high homology to known genes, whereas remaining 17 were novel. A murine thymic cDNA library was screened by using one cDNA fragment that expressed with higher level in total RNA of l-month old murine thymic tissues than 10-montholds. Finally, one 1470 bp fragment was cloned and showed a 99% of homology to murine transketolase.Conclusion Expression of murine thymic genes has displayed a marked difference with age. These genes might be participated in thymic atrophy.关键词
DDRT-PCR小鼠胸腺基因差异表达分类
生物科学引用本文复制引用
李永海,苏华,何维,崔莲仙,越智宏伦..小鼠胸腺增龄相关性基因的差异表达及其克隆[J].中国医学科学院学报,2001,23(2):158-162,5.
