中华医学杂志（英文版）2008，Vol.121Issue(16)：1583-1587,5.
Edaravone attenuates ischemia-reperfusion injury by inhibiting oxidative stress in a canine lung transplantation model
Edaravone attenuates ischemia-reperfusion injury by inhibiting oxidative stress in a canine lung transplantation model
XU Jin-zhi 1SHEN Bao-zhong 2LI Ye 1ZHANG Tong 2XU Wan-hai 2LIU Xiao-wei 1LU Hong-guang1
作者信息
- 1. Department of Thoracic Surgery Fourth Affiliated Hospital of Harbin Medical University,Harbin, Heilongjiang 150001, China
- 2. Department of Medical Imaging Fourth Affiliated Hospital of Harbin Medical University,Harbin, Heilongjiang 150001, China
- 折叠
摘要
Abstract
Background Previous reports have confirmed that edaravone has protective effects against ischemia-reperfusion(IR) injury of many organs.In this study,we investigated the effect of edaravone on preventing IR injury of the lung in a canine lung transplantation model. Methods Twelve weight-matched pairs of random-bred dogs were randomized into two groups.Within each pair,one dog served as donor and the other as recipient.In the study group,prostaglandin EI(PGEl)(8 μg/kg)was injected into the donor pulmonary artery(PA)before occlusion and the donor lungs were flushed with 1.0L of LPD solution containing edaravone(10mg/kg)and stored in the same LPD solution at a temperature of 1.C for 8 hours.The left single lung transplantation was then performed and recipients received intravenous injection with edaravone l 1 0 mg/kg)at the onset of reperfusion.In the control group,edaravone was substituted by the same volume of sterile saline solution.Another six dogs were obtained as normal control group in which left lungs were dissected after thoracotomy without an IR injury.One hour after repeffusion.or after dissection of the left lung,the right lung was excluded from peffusion and ventilation after which,cardiopulmonary parameters were measured.Wet/dry ratios,malondiaIdehyde(MDA)and myeloperoxidase (MPO)levels were assessed and histological analysis of lung tissue performed at the same time.Results All animals survived until the end of the experiment.The study group showed significantly decreased wet/dry ratios(treated:(74.1±4.2)%vs control:(86.8±5.2)%,P<0.01),MDA levels(treated:0.50±0.08 vs control:0.88±0.15,P <0.01)and MPO activity(treated:0.23±0.05 vs control:0.43±0.07,P<O.01)compared to the control group two hours after occlusion of the right side.In the control group,pulmonary vascular resistance(PVR)was increased markedly and arterial oxygen partial pressure deteriorated significantly after exclusion of the right side compared to those in the treatment group.Conclusions Edaravone attenuates IR-induced lung injury and preserves lung function by inhibiting oxidative stress and decreasing leukocyte extravasation in a canine lung transplantation model.关键词
edaravone/ischemia-reperfusion injury/lung transplantation/oxidative stress/reactive oxygen speciesKey words
edaravone/ischemia-reperfusion injury/lung transplantation/oxidative stress/reactive oxygen species分类
医药卫生引用本文复制引用
XU Jin-zhi,SHEN Bao-zhong,LI Ye,ZHANG Tong,XU Wan-hai,LIU Xiao-wei,LU Hong-guang..Edaravone attenuates ischemia-reperfusion injury by inhibiting oxidative stress in a canine lung transplantation model[J].中华医学杂志（英文版）,2008,121(16):1583-1587,5.
