中国临床康复2004，Vol.8Issue(8)：1584-1586,3.
人羊膜与成纤维细胞的共培养在组织工程中的应用
Co-culture of fibroblasts and human amnion in application of tissue engineering
摘要
Abstract
AIM:To observe the growth characteristics of fibroblasts on human amnion(HAM) and the occurrence of collagen fibril by co-culture of fibroblasts and HAM so as to provide a basis for the HAM as the carrier of the skin tissue engineering. METHODS:Various concentration of fibroblasts were seeded onto human amnion extra-cellular matrix(HA-ECM)and cultured in vitro.The performance of cells' growth, array, adhesion and collagenous fibril formation on HA-ECM was observed with light microscope and transmission electron microscope. RESULTS:Fusiformed fibroblasts attached HA-ECM firmly and compactly, the fusiform cells oriented radiantly or longitudinally in the trial group.A lot of fusiform fibroblasts closely packed onto the HA-ECM.When viewed in the tussue slice,under EM they attached firmly and compactly.The basal membrane of fusiform fibroblasts with oval nucleus and rich in chromatin reached much protuberance into matrix of HA-ECM and collagen fibril cumulating outside cells. CONCLUSION:The optimal cell concentration is 3.5× 109L-1,HA-ECM an is ideal carrier for fibroblasts because of its scaffold and diffusion characters.The fibroblasts attached,adhered and proliferated well on the HA-ECM.关键词
人羊膜细胞外基质/成纤维细胞/细胞 ,培养的分类
医药卫生引用本文复制引用
何清义,李起鸿,许建中,杨柳..人羊膜与成纤维细胞的共培养在组织工程中的应用[J].中国临床康复,2004,8(8):1584-1586,3.基金项目
国家自然科学基金重点项目资助(39830100) （39830100）
