水生生物学报2001，Vol.25Issue(2)：159-166,8.
水华蓝藻产毒特性的PCR检测法
CHARACTERIZATION OF TOXIC WATERBLOOM-FORMING CYANOBACTERIA BY MODIFIED PCR
摘要
Abstract
The present study surveyed the distribution ofmicrocystin-producing gene——mcyB by PCR among 38 bloom-forming cyanobacterial strains from FACHB-Collection. The results demonstrated that special amplifications were being gotten only from 19 toxic Microcystis strains, whereas it was deficient in nontoxic ones got none. The PCR results showed highly agreement with the toxicity of every strain determined by HPLC, ELISA, and Bioassay respectively. Furthermore, the traditional PCR protocols were simplified by checking the cyanobacteria cells directly instead of the previously used extracted genomic DNA. The whole cells PCR we developed in this study could be successfully applied to cultured algae materials, water samples and lyophilized cyanobacterial cells. Thus, discrimination of toxic and nontoxic Microcystis strains by molecular biological technologies proves to be practicable and efficacious.关键词
全细胞PCR/水华蓝藻/微囊藻/微囊藻毒素/mcyB/基因Key words
Whole cells PCR/Water-blooming cyanobacteria/Microcystis/Microcystins/mcyB/Gene分类
农业科技引用本文复制引用
潘卉,宋立荣,刘永定,朱运芝,沈强..水华蓝藻产毒特性的PCR检测法[J].水生生物学报,2001,25(2):159-166,8.基金项目
国家自然科学基金(项目号No.39730380) （项目号No.39730380）
国家科技部项目(No. DCH-01-001) （No. DCH-01-001）
