眼科学报2000，Vol.16Issue(1)：1-6,6.
Preliminary Study on in Vitro Induced Differentiation of Embryonic Stem Cells into Neurons
Preliminary Study on in Vitro Induced Differentiation of Embryonic Stem Cells into Neurons
摘要
Abstract
Purpose: To study preliminarily in vitro induced differentiation of embryonic stem cells into neurons for further investigation of an alternative for the treatment of glaumatous neuropathy.Materials and methods: Supernatant of cultured Buffalo rat liver cells (buffalorat liver cell-conditioned medium, BRL-CM) was used for culturing embryonic stem cells (ES-D3 cell line). Morphological features of undifferentiated ES cells were studied by HE staining and electron microscopy. Based on the methods used by Bain et al, we modified the methods and used retinoic acid (RA) as an inducer to differentiate ES-D3 cells and cytosine arabinoside (Ara-C) as inhibitor of proliferative cells. The growth of the cells was observed under phase contrast microscope.Results: ES-D3 cells cultured by BRL-CM grew in aggregates and remained undifferentiated. Electromicroscopy showed large nucleus and a large amount of mitochondria in undifferentiated ES cells and many processes on the surfaces. In the first day after the adding of retinoic acid, some neuron-like cells with one, two or more processes were present. In the second day after adding RA and the first day after the plus of 10μm Ara-C, a large amount of neuron-like cells appeared, with the formation of neuron-like networks.Conclusions: Combined use of RA and Ara-C can induce ES cells to differentiate into neuron-like cells. Our present preliminary study might provide insights into an alternative for the treatment of glaucomatous neuropathy by the transplantation of embryonic stem cells. Eye Science 2000; 16: 1 ～ 6.关键词
embryonic stem cells/differentiation/neuron/glaucomaKey words
embryonic stem cells/differentiation/neuron/glaucoma引用本文复制引用
Jian Ge,Yan Guo,Zhichong Wang,Haichuan Liu,Zhikuan Yang,Yongping Li,Shunong Li..Preliminary Study on in Vitro Induced Differentiation of Embryonic Stem Cells into Neurons[J].眼科学报,2000,16(1):1-6,6.基金项目
This paper is granted by 973 Branched Project(Gl999054301 ),National Natural Sciences Foundation of China (No. 39770789),Natural Sciences Foundation of Guangdong Province (No. 990092)and 211 Project Foundation (No. 98010). （Gl999054301 ）
