林产化学与工业2004，Vol.24Issue(4)：1-6,6.
碳源和氮源对白腐菌Sarcodon asparatus合成脂肪酶的影响
EFFECTS OF CARBON AND NITROGEN SOURCES ON LIPASE PRODUCTION BY SARCODON ASPARATUS
摘要
Abstract
Carbon sources and nitrogen sources affecting lipase production by Sarcodon asparatus were investigated.Lipase activity was the highest(0.65 U/mL) for olive oil compared with other carbon sources.Lipase production was stimulated by peptone(0.65 U/mL),urea(0.67 U/mL) and ammonium chloride(0.62 U/mL),and repressed by ammonium sulfate with a low lipase activity of 0.08 U/mL.C/N ratio was a significant factor for lipase production and cell growth.When C/N ratio was raised from 2 to 10,lipase activity decreased from 0.65 to 0.14 U/mL,and biomass increased from 3.08 to 4.58 mg/mL.The optimal culture conditions of S.asparatus were pH value 5.5 and 28 ℃,and lipase activity was 0.65 U/mL with a biomass concentration of 3.08 mg/mL.关键词
脂肪酶/碳源/氮源/白腐菌/Sarcodon asparatusKey words
lipase/carbon source/nitrogen source/white rot fungi/Sarcodon asparatus分类
生物科学引用本文复制引用
勇强,姜镇河,余世袁..碳源和氮源对白腐菌Sarcodon asparatus合成脂肪酶的影响[J].林产化学与工业,2004,24(4):1-6,6.基金项目
国家自然科学基金资助项目(30100142) （30100142）
