中国药理学与毒理学杂志2006，Vol.20Issue(1)：1-6,6.
牛磺酸对皮质神经元急性氧糖缺失所致损伤的保护作用
Neuroprotective effect of taurine against acute cortical neuron injury induced by oxygen-glucose deprivation
摘要
Abstract
AIM Taurine was reported neuroprotective under several ischemic models in vivo. In this study, the direct effect of taurine against oxygen-glucose deprivation (OGD) inducing acute neuronal injury and the underlying mechanisms in vitro were investigated. METHODSFour hours OGD was used to induce in vitro ischemic injury in rat cortical neurons. Taurine 5, 10 and 20 mmol·L-1 was added 20 h before and during 4 h OGD period respectively. Mortality rate of neuron was assayed by MTT and flow cytometry methods. Level of neuronal [Ca2+]i was detected by Fura 2/AM loading. Amino acid concentrations in culture media were measured by high performance liquid chromatography. RESULTS Under OGD conditions, neuronal death was markedly increased, and the levels of neuronal [Ca2+]i and extracellular glutamate level were enhanced obviously. Taurine pretreatment obviously decreased the percentage of neuronal death induced by OGD. In addition, abnormal elevation of neuronal [Ca2+]i and extracellular glutamate level induced by OGD both were markedly repressed by taurine. CONCLUSION Taurine can alleviate rat cortical neuron injury induced by OGD, the mechanisms were likely due to repressing calcium overload and inhibiting excessive release or leakage of glutamate under such conditions.关键词
牛磺酸/大脑皮质/神经元/氧糖缺失/钙,细胞内/谷氨酸/细胞凋亡Key words
taurine/cerebral cortex/neurons/oxygen glucose deprivation/calcium, cytosolic/glutamic acid/apoptosis分类
医药卫生引用本文复制引用
罗璨,郭莲军,戴支凯,吴芹,石京山..牛磺酸对皮质神经元急性氧糖缺失所致损伤的保护作用[J].中国药理学与毒理学杂志,2006,20(1):1-6,6.基金项目
国家自然科学基金资助项目(30171082)The project supported by National Natural Science Foundation of China (30171082) （30171082）
