肿瘤防治研究2001，Vol.28Issue(3)：161-163,3.
绿色荧光蛋白转染胶质瘤细胞的体内实验研究
In Vivo Experiment on Brain Glioma Cells Transfected with Green Fluorescent Protein
摘要
Abstract
Objective To determine whether fluorescence from C6 glioma cellstransfected with the enhanced green fluorescent protein gene in vitro and xenotransplanted into the brain of SD rats would permit the detection of brain tumor invasion in vivo. Methods C6 glioma cells were transfected with a plasmid vector (pEGFP\|N3) containing the EGFP gene. Stable EGFP\|expressing clone were isolated and stereotactically injected into the parenchyma of rats. Four weeks later,rats were killed and continuous brain sections were examined using fluorescence microscopy after adjacent sections were performed by immunohistochemistry or routine HE staining for detection of tumor cell invasion. Results We demonstrate that EGFP-transfected C6 glioma cells maintain stable high-level EGFP expression in the central nervous system during their growth in vivo. EGFP fluorescence clearly demarcated the primary tumor margins and readily allowed for the visualization of distant micrometastasis and local invasion on the single-cell level. Small locally invasive foci, including those immediately adjacent to the leading invasive edge of the tumor, were virtually undetectable by routine HE and immunohistochemistry staining. Conclusion We showed that EGFP\|transfected C6 glioma cells can be visualized by fluorescence microscopy after intracranial implantation. This method is superior to routine HE staining and immunohistochemistry for the detection and study of relevant patterns of brain tumor invasion in vivo.关键词
胶质细胞瘤/绿色荧光蛋白/侵袭分类
医药卫生引用本文复制引用
李侠,章翔,吴景文,高大宽,刘先珍,梁景文,王煊..绿色荧光蛋白转染胶质瘤细胞的体内实验研究[J].肿瘤防治研究,2001,28(3):161-163,3.基金项目
国家自然科学基金资助项目(39970752) （39970752）
