中国现代医学杂志2005，Vol.15Issue(2)：161-164,4.
参附对大鼠肠缺血再灌注时肠上皮细胞Caspase-3,Bcl-2基因表达的影响
Study of Shenfu injection on expression of Caspase-3 and Bcl-2 of intestinal mucosal epithelial cells during ischemia-reperfusion in rats
摘要
Abstract
[Objective] To investigate effects of ShenFu injection on apoptosis of intestinal mucosal epithelial cells during ischemia-reperfusion in rats. and discuss the mechanisms. [Methods] Ischemia-reperfusion model was established with rats, twenty-four rats were divided randomly into control group (C group), ischemia-reperfusion group(I/R group) and ShenFu injection (SF group). At choiced time rat guts were cut to sections for HE, Tunnel staining and caspase-3, Bcl-2 protein immunohistochemical staining. [Results] In the detection of apoptosis cells the number increased more notably in I/R group, it was decreased more in SF group than I/R group and it had increased more than C group (P ＜0.05). The expression of caspase-3 in I/R group was increased more notably than C group and SF group (P ＜0.01), it hadn't distinct difference between C group and SF group; the expression of Bcl-2in SF group increased more markedly than I/R group (P ＜0.05), the expression in I/R group was less than C group (P＜0.01) . The injury of intestinal mucosal in SF group alleviated more than I/R group. [Conclusions] Shenfu injection can reduce the apoptosis of intestinal mucosal epithelial cells during ischemia-reperfusion by restraining the expression of caspase-3 gene and accelerating the expression of Bcl-2 gene, thus to alleviate the injury of intestinal mucosal epithelial cells during ischemia-reperfusion.关键词
参附注射液/再灌注损伤/小肠/凋亡/Caspase-3/Bcl-2Key words
Shenfu injection/ischemia-reperfusion/intestinal/apoptosis/caspase-3/Bcl-2分类
医药卫生引用本文复制引用
孟庆涛,夏中元,贾一帆,刘先义,陈向东,熊桂先..参附对大鼠肠缺血再灌注时肠上皮细胞Caspase-3,Bcl-2基因表达的影响[J].中国现代医学杂志,2005,15(2):161-164,4.基金项目
Supported by Education Department Foundation of Hubei Province,NO.2003X125 （）
