中国药理学与毒理学杂志2009，Vol.23Issue(3)：161-167,7.DOI:10.3867/j.issn.1000-3002.2009.03.001
钩藤碱对麻醉大鼠颈动脉窦压力感受器活动的抑制作用
Inhibition of rhynchophylline on carotid sinus baroreceptor activity in anesthetized rats
摘要
Abstract
AIM To elucidate the effect of rhynchophylline(Rhy) on carotid sinus baroreceptor activity (CBA). METHODS By recording sinus nerve afferent discharge activity with isolated carotid sinus perfusion, parameters of CBA, such as peak slope (PS), peak integral value (PIV), threshold pressure (TP) and saturation pressure (SP) were examined. ①Rhy 10, 50, and 100 μmol·L-1, dissolved in K-H solution, was perfused into isolated carotid sinus, then the effects of Rhy on parameters of CBA were observed while intrasinus pressure was altered in a stepwise manner. ②NG-nitro-L-arginine methyl ester (L-NAME) 10 mmol·L-1, tetraethylammonium (TEA) 1 mmol·L-1 and Bay K8644 500 nmol·L-1 were perfused into isolated carotid sinus, and effects of them on the response of carotid baroreceptor to Rhy were observed. RESULTS ① By perfusing the isolated carotid sinus with Rhy 10 μmol·L-1, PS decreased from (19.2±0.3)% to (18.2±0.1)%·kPa-1and the PIV decreased from (219.3±3.3)% to (199.1±3.8)%, while TP and SP increased from (8.2±0.3) to (9.1±0.1)kPa and (21.5±0.1) to (22.1±0.1)kPa, respectively. By perfusing with Rhy 50 and 100 μmol·L-1, the changes in PS, TP and SP were in concentration-dependent manner, and this indicated inhibitory effect of Rhy on CBA. ②Pretreatment with L-NAME 100 μmol·L-1 did not affect inhibitory action of Rhy 50 μmol·L-1 on CBA. ③Pretreatment with TEA 1 mmol·L-1 had no effect on inhibitory effect of Rhy 50 μmol·L-1 on CBA. ④Pretreatment with Bay K8644 500 nmol·L-1 could mostly attenuate effect of Rhy 50 μmol·L-1 on CBA. CONCLUSION Rhy inhibits CBA via blocking calcium influx in baroreceptor nerve ending.关键词
钩藤碱/颈动脉窦/压力感受器/Bay/K8644Key words
rhynchophylline/carotid sinus baroreceptor/Bay K8644分类
医药卫生引用本文复制引用
刘宜先,董京辉,高璐,马慧娟,武宇明,张翼,何瑞荣..钩藤碱对麻醉大鼠颈动脉窦压力感受器活动的抑制作用[J].中国药理学与毒理学杂志,2009,23(3):161-167,7.基金项目
河北省科技厅课题(062761773)资助项目 （062761773）
河北省教育厅课题(Z2006110)资助项目Foundation item: The project supported by Foundation of Science & Technology Bureau of Hebei Province(062761773) （Z2006110）
and Natural Science Foundation of Education Bureau of Hebei Province(Z2006110) （Z2006110）
