中风与神经疾病杂志2001，Vol.18Issue(1)：16-18,3.
大鼠单侧大脑中动脉栓塞后脑内乙酰胆碱酯酶的光镜与电镜酶组化研究
Study on dynamic changes of acetylcholine esterase(AChE) after middle cerebral artery occlusion with light and electron microscope
摘要
Abstract
Objective To study the dynamic changes of cholinergic system after cerebral infarction. Methods A rat model of right middle cerebral artery occlusion(MCAO) was made by insertion of an intraluminal suture. The enzyme histochemistry study of Acetylcholine esterase (AChE) was done at 0.5h、1h、2h、3h、6h respectively after MCAO under light microscope. The activity changes of AChE under electron microscope were also done. Results Under light microscope the activity of AChE began to change at 1h after MCAO, showing the weakening of staining priority in the fourth cell layer, and continued to decrease with MCAO time extending from 2h after MCAO. Under electron microscope, the AChE enzyme particles were located in the processes、the anterior and posterior membranes of the synapses which decreased both in quantity and density in the ischemic penumbra after MCAO, and almost disappeared in the infarcted core. Conclusion The activity of AChE changes before morphologic changes appeared after MCAO.The cholinergic system become abnormality in the early stage after cerebral infarction.关键词
乙酰胆碱酯酶/酶组织化学/大脑中动脉栓塞分类
医药卫生引用本文复制引用
谭斐,金武,牛世贵..大鼠单侧大脑中动脉栓塞后脑内乙酰胆碱酯酶的光镜与电镜酶组化研究[J].中风与神经疾病杂志,2001,18(1):16-18,3.
