分析测试学报2001，Vol.20Issue(1)：16-19,4.
乳糖和麦芽糖水解反应速率常数的毛细管电泳测定方法
Determination of Reaction Rate Constant of Lactose and Maltose Hydrolysis by Capillary Electrophoresis with Electrochemical Detection
摘要
Abstract
The concentration of lactose and maltose and their hydrolysisproducts were monitored by ca-pillary electrophoresis-electrochemical detection due to its high resolution and high sensitivity of detection. The reaction rate constant of lactose and maltose hydrolysis at different temperature in 0.75 mol/L H2SO4 were obtained from the concentration change of lactose and maltose during the process of hydrolysis. At 85 ℃ , 90 ℃ and 95 ℃ , the measured rate constants of lactose hydrolysis were 13.3× 10-3 min-1, 30.1× 10-3 min-1 and 52.5× 10-3 min-1, respectively, from which the activation energy of the reaction(Ea) value of 161.1 kJ/mol was derived. At 85 ℃ , 95 ℃ and 100 ℃ , the measured rate constants of maltose hydrolysis were 17.7× 10-3 min-1, 84.6×10-3 min-1 and 150× 10-3 min-1, respectively, Ea value obtained was 194.7 kJ/mol.关键词
毛细管电泳/电化学检测/乳糖/麦芽糖/反应速率常数/水解分类
化学化工引用本文复制引用
张欣,丁祥欢,朱雪焱,叶建农..乳糖和麦芽糖水解反应速率常数的毛细管电泳测定方法[J].分析测试学报,2001,20(1):16-19,4.基金项目
中国科学院长春应用化学研究所电分析化学开放实验室资助项目 （）
