林业科学2003，Vol.29Issue(2)：162-167,6.
7个桉树杂交亲本RAPD位点多态性和杂合性的研究
DNA POLYMORPHISM AND HETEROZYGOSITY IN SEVEN PARENTAL EUCALYPT CLONES AS REVEALED BY RAPD MARKERS
摘要
Abstract
Five controlled crosses of Eucalyptus urophylla S.T.Blake×E.tereticornis Smith were used for RAPD analysis on DNA polymorphism and heterozygosity of the seven parental clones.A total of 58 fragments were amplified with 9 single arbitrarily chosen primers,and 42 fragments(72.4%) were scored as polymorphic among the parents.The genetic distance (GD) between parents was calculated with RAPD data matrix.High levels of heterozygosity were revealed in the parents studied,with an average 28.0%,but the percentage of heterozygous loci varied with parent from 16.2% to 39.5%.The implications for material selection in genetic linkage map construction were discussed.关键词
RAPD/多态性/杂合性/桉树Key words
RAPD/Polymorphism/Heterozygosity/Eucalypt species分类
农业科技引用本文复制引用
甘四明,施季森,白嘉雨,吴坤明,吴菊英..7个桉树杂交亲本RAPD位点多态性和杂合性的研究[J].林业科学,2003,29(2):162-167,6.基金项目
国家自然科学基金项目(39870619)和国际科学基金项目(International Foundation for Science,IFS)(D/2947-1). （39870619）
