海洋科学2009，Vol.33Issue(7)：16-22,7.
超低温冷冻对长鳍篮子鱼精子中几种酶活性的影响
Effects of cryopreservation on enzyme activity of Siganus canaliculatus spermatozoa
摘要
Abstract
This paper studied the effects of cryopreservation(-196℃) on the enzyme activity(total ATP,CK,SDH,LDH,SOD, CAT, GSH-PX and GR) in Siganus canaliculatus spermatozoa. The changes of enzyme activity were analysed in the spermatozoa using the reagent box respectively. The results showed the activities of spermatozoa dropped after cryopreservation, GR activities increased significantly(P<0.05), the activities added from 163.54 U/L±17.14 U/L to 239.33 U/L±22.24 U/L, other seven kinds of enzyme activities descended significantly(P<0.05).The activities of total ATP, CK, SDH dropped from 9.20 U/mL±0.28 U/mL,25.59 U/mL±0.13 U/mL and 18.33 U/mL±2.52 U/mL to 4.18 U/mL±0.81 U/mL,17.87 U/mL±0.56 U/mL and 9.33 U/mL±1.53 U/mL respectively,the activities of LDH, SOD and CAT declined from 14 828.66 U/L±866.56 U/L,421.68 U/mL±12.66 U/mL and 121.17 U/mL±15.04 U/mL to 11324.33 U/L±689.58 U/L,199.56 U/ml±50.55 U/ml and 74.60 U/mL±12.70 U/mL canaliculatus.关键词
超低温冷冻/长鳍篮子鱼(Siganus canaliculatus)/精子/酶活性Key words
cryopreservation/ Siganus canaliculatus/ spermatozoa/ enzyme activity分类
农业科技引用本文复制引用
黄晓荣,章龙珍,庄平,江琪,刘鉴毅,姚志峰,赵峰,张涛,冯广朋..超低温冷冻对长鳍篮子鱼精子中几种酶活性的影响[J].海洋科学,2009,33(7):16-22,7.基金项目
国家科技支撑计划项目(2006BAD03B08-08) （2006BAD03B08-08）
国家科技基础条件平台项目(2006DKA30470_004) （2006DKA30470_004）
中央级公益性科研院所基本科研业务费专项资金项目(2007T02) （2007T02）
