中华医学杂志（英文版）2002，Vol.115Issue(11)：1624-1627,4.
支气管哮喘患儿IL-4近侧启动子区克隆和多态性分析
Cloning and polymorphism analysis of IL-4 proximal promoter in asthmatic children
周玉峰 1付劲蓉 1吴健民 2李成荣1
作者信息
- 1. 深圳市儿童医院儿科研究所分子免疫学实验室,深圳,518026
- 2. 华中科技大学同济医学院附属协和医院检验科分子生物学实验室,武汉,430022
- 折叠
摘要
Abstract
Objective To clone and study the polymorphism within interleukin-4 (IL-4) proximal promoter of asthmatic children. Methods The IL-4 proximal promoter segments were amplified and selected by polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP) with genomic DNA from ten healthy children and forty patients with dominantly allergic familial histories as templates. The selected PCR segments were cloned into recombinant plasmids pIL-4-Jx2. The PCR inserts were sequenced by dideoxy chain termination method. Results Seven aberrant bands were found in SSCP analysis from forty asthmatic patients. The sequencing results showed that four variant sites were found within or adjacent to the known IL-4 regulatory element. A C to A transversion located at -229 position was just within the positive regulatory element-Ⅰ (PRE-Ⅰ) in one patient. A C to T transition adjacent to the negative regulatory element-Ⅱ (NRE-Ⅱ) and an extra G adjacent to TATA box were found in two patients. A five base nucleotide deletion was found near signal transducers and activators of transcription-6 responsive element (STAT-6 RE) in one patient. Conclusion There were polymorphisms within the IL-4 proximal promoter of allergic asthmatic patients and these polymorphisms might result in aberrant expression of IL-4 gene and asthma.关键词
哮喘/白介素-4/启动子/分子克隆/序列测定Key words
asthma/interleukin-4/promoter/molecular cloning/sequencing分类
医药卫生引用本文复制引用
周玉峰,付劲蓉,吴健民,李成荣..支气管哮喘患儿IL-4近侧启动子区克隆和多态性分析[J].中华医学杂志（英文版）,2002,115(11):1624-1627,4.
