西北国防医学杂志2001，Vol.22Issue(2)：163-164,2.
Fas／APO－1基因真核表达质粒的构建
Construction of eukaryotic expression plasmid of Fas／APO－1 gene
张德海 1肖冰2
作者信息
- 1. 兰州军区兰州总医院胸心外科,
- 2. 第一军医大学消化内科研究所,
- 折叠
摘要
Abstract
Objective:To construct eukaryotic expression plasmid of Fas／APO－1 gene for further study on Fas／APO－1 function in apoptosis of cancerous cells.Methods:The plasmids pBluscript-Fas cDNA were prepared by double digestion;then the DNA fragment was extracted,purified and ligated with T4 ligase in the plasmid pBK-CMV to form pBK-Fas cDNA.Results:The original and reconstructed plasmids were found to contain correct-sized Fas／APO－1 cDNA fragment by agarose gel electrophoresis after digestion.Conclusion:A eukaryotic expression plasmid of Fas／APO－1 gene was constructed successfully.关键词
基因/Fas／APO－1/真核表达载体分类
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张德海,肖冰..Fas／APO－1基因真核表达质粒的构建[J].西北国防医学杂志,2001,22(2):163-164,2.
