河南医科大学学报2001，Vol.36Issue(2)：163-165,3.
地高辛标记探针原位杂交法检测人脑胶质瘤组织猴病毒40的表达
Expression of simian virus 40 DNA in human brain glioma tissues
摘要
Abstract
Aim: To detect the location of simian virus 40 (SV40) DNA and its probable biological function in oncogenesis of human brain gliomas.Methods:A total of 256 cases of human brain glioma tissues and 11 cases of normal brain tissues were studied for SV40 DNA by digoxin labeled probe in situ hybridization.Results:Hybridization signals were detected within the nuclei of glioma cells and the positive cells distributed diffusely or focally.SV40 DNA positivity rate was 40.6% in brain glioma tissues,whereas none of the normal tissues had positive results.The correlation brain between SV40 DNA positivity rate and pathological degree of tumor was not apparent(P＞0.05).Conclusion:SV40 infection is closely related to etiology of glioma.The technique of digoxigenin labeled probe in situ hybridization is a new specific method in studying the location,distribution and infection mechanisms of SV40 DNA.关键词
胶质瘤/猴病毒40/DNA分类
医药卫生引用本文复制引用
陈东,步星耀,高冬玲,姜国忠,张云汉..地高辛标记探针原位杂交法检测人脑胶质瘤组织猴病毒40的表达[J].河南医科大学学报,2001,36(2):163-165,3.基金项目
河南省杰出青年基金资助项目 （）
