首页|期刊导航|南京医科大学学报(英文版)|Dephosphorylation of cardiomyocyte Cx43 is associated with myocardial ischemia and reperfusion injury
南京医科大学学报(英文版)2009，Vol.23Issue(3)：163-167,5.
Dephosphorylation of cardiomyocyte Cx43 is associated with myocardial ischemia and reperfusion injury
Dephosphorylation of cardiomyocyte Cx43 is associated with myocardial ischemia and reperfusion injury
摘要
Abstract
Objective:Myocardial ischemia/reperfusion(I/R) injury is the leading cause of death in the world. However, the details of the mechanism of its pathophysioiogy are still unknown. The present study was designed to investigate the role of connexin 43(Cx63) in acute models of myocardial I/R injury. Methods: Male C57BL/6 mice were subjected to myocardial ischemia(45 rain) followed by reperfusion(4 hrs) in vivo. The whole operation was monitored using a two-lead ECG. Hearts were harvested and the level of protein was assessed by western blot analysis. Haematoxylin and Eosin(HE) staining was used to detect the extent of neutrophil infiltration. The expression level of IL-6 was detected by ELISA. Results: A murine myocardial I/R injury model was constructed successfully. Phosphorylated Cx43 decreased 83.45% while non-phosphorylated Cx43 increased 1.62- fold in the myocardium after I/R injury. Neutrophil infiltration and the expression of the inflammatory cytokine IL-6 increased in the myocardium following I/R. Conclusion: During myocardial I/R injury, cardiomyocyte Cx43 is dephosphorylated, and this may be associated with an inflammatory response.关键词
myocardium/ I/R injury/ connexin 43/ inflammatory responseKey words
myocardium/ I/R injury/ connexin 43/ inflammatory response分类
临床医学引用本文复制引用
Zhijuan Cao,Xuan Xu,Linli Que,Qi Chen,Yuehua Li..Dephosphorylation of cardiomyocyte Cx43 is associated with myocardial ischemia and reperfusion injury[J].南京医科大学学报(英文版),2009,23(3):163-167,5.基金项目
This work was supported by Jiangsu Provincial Science Foundation of China (BK2006229). （BK2006229）
