中国药房2009，Vol.20Issue(21)：1633-1635,3.
微乳TLC法分离鉴定肠激安方中多种有效成分
Mieroemulsion Thin - layer Chromatography in the Separation and Identification of Various Effective Compositions in Changji'an Prescription
摘要
Abstract
OBJECTIVE: To establish the microemulsion TLC for the separation and identification of various effective compositions in Changji'an prescription. METHODS: The compositions in Changji'an prescription were identified on polyam-ide film(fixed phase) with five distribution ratios of sodium lauryi sulphate - n- butanol - skellysolve C- H2O microemulsions as developing agents. The effects of factors such as the water content of the microemulsion and the temperature on the sepa-ration efficacy were studied. RESULTS: With microemulsion(75% water content) as developing agent, Rhizoma Coptidis in Changji' an prescription was detected; with microemulsion (75% water content) - formic acid (9.0 : 1.0) as developing agent, Corydalis yanhusuo, Saposhnikovia divaricata, chlorogenic acid and Radix et Rhizoma Glycyrrhizae in Changji'an prescription were detected. CONCLUSION: Microemulsion TLC is simple, environmental- friendly, sensitive with ideal separation effi-cacy, and it can be used for the separation and identification of various compositions in Chinese patent medicines.关键词
微乳/薄层色谱法/肠激安方/分离/鉴定Key words
Microemulsion/TLC/Changji'an prescription/Separation/Identification分类
医药卫生引用本文复制引用
唐洪梅,闫雪,李得堂..微乳TLC法分离鉴定肠激安方中多种有效成分[J].中国药房,2009,20(21):1633-1635,3.基金项目
广东省医院药学研究基金(2008A016) （2008A016）
广州市科技计划项目(2005J1-C0201) （2005J1-C0201）
