生态环境2007，Vol.16Issue(6)：1633-1636,4.
不同氮浓度下三角褐指藻生长特性和化学组成
Growth characteristics and chemical compositions of Phaeodactylum tricornutum under different nitrogen concentrations
摘要
Abstract
The marine diatom Phaeodactylum tricornutum is a phytoplankton species that contains a high content of bioactive compounds and thus it can be served as an important food source and feed additive in the successful rearing of aquatic animals like shrimp and fish.While its overproliferation along the coastal areas of China in recent years has brought about many negatively environmental and economic consequences around.In order to investigate the effects of different nitrogen concentrations on the growth characteristics and chemical compositions in Phaeodactylum tricornutum, algal cell density, specific growth rate, biomass, and the contents of soluble sugar, protein and chlorophyll a have been examined in laboratory cultures by employing low nitrogen (LN, 44 μmol·L-1 NaNO3), medium nitrogen (MN, 880 μmol·L-1 NaNO3) and high nitrogen (HN, 4400 μmol·L-1 NaNO3) concentrations.Our results indicated that the growth curve, specific growth rate and cell biomass of Phaeodactylum tricornutum were significantly affected by nitrogen concentrations.They were 149.50×104 cells·mL-1, 0.37 d-1, and 209.33 μg·mL-1 under LN condition, whereas they were 951.67×104 cells·mL-1, 0.67 d-1, and 806.00 μg·mL-1 under HN condition at the 10th day during the stationary phase.Although HN treatment significantly enhanced the algal cell number and cell yield in the stationary phase, it did not show remarkable differences in the growth curve and specific growth rate in the early stages as compared with MN treatment.Besides, the contents of soluble sugar, protein and chlorophyll a were obviously increased with the increasing of nitrogen concentrations, which were approximately 2.5, 1.5 and 15 times higher in HN treatment than those in LN treatment, respectively.It can be concluded from the present investigation that higher nitrogen concentrations can accelerate the growth and division as well as biosynthesis of some chemical compositions of Phaeodactylum tricornutum, and therefore this should be taken into account for the prevalence of red tides dominated by this marine microalga in recent years.关键词
氮浓度/生长特性/化学组成/三角褐指藻Key words
nitrogen concentrations/ growth characteristics/ chemical compositions/ Phaeodactylum tricornutum分类
资源环境引用本文复制引用
蔡卓平,段舜山..不同氮浓度下三角褐指藻生长特性和化学组成[J].生态环境,2007,16(6):1633-1636,4.基金项目
国家自然科学基金项目(30370231) （30370231）
