国际眼科杂志2009，Vol.9Issue(9)：1641-1645,5.DOI:10.3969/j.issn.1672-5123.2009.09.001
(R,R)-XY-10和(S,S)-XY-10 诱导的视网膜色素上皮细胞增生作用及其作用机制
Proliferation of retinal pigment epithelial cells induced by (R,R)-XY-10 and (S,S)-XY-10 and their action mechanisms
Yu-Wen Cheng 1王玉良 2张奕华 2彭司勋 2George C Y Chiou1
作者信息
- 1. 美国德州大学城,德州农工大学医学院,眼科药理学研究所
- 2. 210009,中国江苏省南京市,中国药科大学新药研究中心
- 折叠
摘要
Abstract
AIM: To investigate the mechanism of proliferation effect induced by (R,R)-XY-10 and (S,S)-XY-10 on retinal pigmented epithelial cells(ARPE-19).METHODS: Human retinal pigmented epithelial cells(ARPE-19) and human umbilical vein endothelial cells (HUVECs) were used to investigate the effect of (R,R)-XY-10 and (S,S)-XY-10 on cell growth,and their mechanisms of proliferative action by using ERK、 AKT、PI3K、Protein kinase C (PKC)and Nitric oxide synthase (NOS) inhibitors.RESULTS: (R,R)-XY-10 and (S,S)-XY-10 dose-dependently increased ARPE-19 cell proliferation,but not on HUVECs. When treated with proliferative inhibitors,H7(5μmol/L)、hypericin(20μmol/L)、PD98059(2μmol/L)、LY294002(50μmol/L)、SH-5 (10μmol/L) and L-NAME (100μmol/L),the proliferative effect was reduced by H7、hypericin、PD98059 and LY294002,but not by SH-5 and L-NAME.CONCLUSION: (R,R)-XY-10 and (S,S)-XY-10 can induce cell proliferation through MAPK and PI3K dependent pathway. KEYWORDS: age-related macular degeneration; (R,R)-XY-10; (S,S)-XY-10; ARPE-19 cells; human umbilical vein endothelial cells; proliferation关键词
年龄相关性黄斑变性/(R,R)-XY-10/(S,S)-XY-10/ARPE-19 cells/人脐静脉血管内皮细胞/增生引用本文复制引用
Yu-Wen Cheng,王玉良,张奕华,彭司勋,George C Y Chiou..(R,R)-XY-10和(S,S)-XY-10 诱导的视网膜色素上皮细胞增生作用及其作用机制[J].国际眼科杂志,2009,9(9):1641-1645,5.
