首页|期刊导航|中华医学杂志（英文版）|Efficiency of adenoviral vector mediated CTLA4Ig gene delivery into mesenchymal stem cells
中华医学杂志（英文版）2003，Vol.116Issue(11)：1649-1654,6.
Efficiency of adenoviral vector mediated CTLA4Ig gene delivery into mesenchymal stem cells
Efficiency of adenoviral vector mediated CTLA4Ig gene delivery into mesenchymal stem cells
摘要
Abstract
Objective To prevent Graft-versus-host disease (GVHD) in rat model, we evaluated the feasibility of mesenchymal stem cells (MSCs) as a gene transfer target and studied the efficiency of recombinant adenovirus mediated gene therapy. Methods We constructed the recombinant adenovirus containing CTLA4Ig gene. Rat MSCs of passages 3-5 were infected by the adenovirus, and the transfection efficiency was monitored by GFP markers. We performed flow cytometric analysis, immunohistochemical and Western blotting analysis to identify the CTLA4Ig expression. The gene transferred MSCs were tested for their ability to inhibit the allogeneic lymphocyte response in vitro and to prevent GVHD in a rat model. Results Recombinant adenovirus pAd-CTLA4Ig was correctly constructed and confirmed. After MSCs were infected by the adenovirus, the CTLA4Ig protein was detected not only in transgenic MSCs, but also in the culture medium. In a mixed lymphocytes response (MLR) test, the transgenic MSCs could significantly inhibit the allogeneic lymphocyte response compared with the control groups (P<0.05). A model of GVHD was developed by transplanting bone marrow cells and spleen lymphocytes of F344 rats to lethally irradiated SD rats. The onset of GVHD could be ameliorated or prevented by co-administration of transgenic MSCs. All the rats in the control groups suffered severe acute GVHD. CTLA4Ig expression was observed in the liver, intestine, kidney and spleen 30 days post- transplantation. Conclusions Our results indicate that adenoviral vectors could efficiently transfer CTLA4Ig gene into MSCs and sustain long-term stable expression in vitro and in vivo.关键词
CTLA4Ig * adenoviral vector * mesenchymal stem cells * mixed lymphocytes responseKey words
CTLA4Ig * adenoviral vector * mesenchymal stem cells * mixed lymphocytes response分类
医药卫生引用本文复制引用
邓宇斌,郭小荑,原清涛,李树浓..Efficiency of adenoviral vector mediated CTLA4Ig gene delivery into mesenchymal stem cells[J].中华医学杂志（英文版）,2003,116(11):1649-1654,6.基金项目
This work was supported by China-France Collaboration Fund (PRA B01-07) and Natural Science Fund of Guangdong Province, China (A101689). （PRA B01-07）
