高等学校化学学报2004，Vol.25Issue(z1)：165-166,2.
Studies on Enzyme Kinetics by Microchip and Related Techniques
Studies on Enzyme Kinetics by Microchip and Related Techniques
James J. Bao 1WANG Huai-Feng 1ZHOU Da-Wei 1Ken R. Wehmeyer2
作者信息
- 1. College of Pharmaceuticals, Tianjin University, Tianjin 300072, China
- 2. Procter & Gamble Pharmaceuticals, 8700 Mason-Montgomery Road, Mason, OH 45040, USA
- 折叠
摘要
Abstract
Both conventional and microchip-based capillary electrophoresis(CE) technologies have been used for the analysis of enzymes. Practical procedures of using CE to determine the Km and Vmax values of an enzyme have been developed. By studying the inhibition to the enzyme, it is possible to select a suitable drug candidate. When compared with the conventional CE method, single lane microchip-based method can improve the speed for the assay three times. By using multiple lane-based microchip, the speed can be further increased.关键词
Enzyme/Enzyme kinetics/Inhibition/Enzyme activityKey words
Enzyme/Enzyme kinetics/Inhibition/Enzyme activity分类
化学化工引用本文复制引用
James J. Bao,WANG Huai-Feng,ZHOU Da-Wei,Ken R. Wehmeyer..Studies on Enzyme Kinetics by Microchip and Related Techniques[J].高等学校化学学报,2004,25(z1):165-166,2.
