中德临床肿瘤学杂志(英文版)2003，Vol.2Issue(3)：166-168,3.
Caspase-3在脐血CD34+造血干/祖细胞中的表达及其意义
Expression of Caspase-3 in Cord Blood CD34+ Cells during Culture in vitro
摘要
Abstract
Objective: To investigate the expression and significance of caspase-3 protein in CD34+ cellsfrom cord blood (CB) during culture in vitro with different growth factors. Methods: RT-PCR, Westernblot and flow cytometry techniques were used to detect the expression of caspase-3 in CD34+ CB cellsduring culture in vitro. Results: Caspase-3 mRNA was constitutively expressed at a low level in freshlyisolated CD34+ cells. The expression of caspase-3 mRNA and protein was upregulated when these cellswere first expanded in suspension culture with growth factors for 3 days. However, only the 32 kDa inactivecaspase-3 proenzyme was detected in the freshly isolated CD34+ cells as well as during the first 3 daysexpansion with cytokines. With longer culture time in vitro, especially in the presence of the combinationof IL-3, IL-6 and GM-CSF, caspase-3 was activated and a cleavage product of 20 kDa became detectable.Conclusion: Caspase-3 is involved in apoptosis of primitive CB CD34+ cells during expansion in vitro.关键词
Caspase-3/CD34+细胞/脐血/凋亡Key words
caspase-3/CD34+ cells/cord blood/apoptosis分类
医药卫生引用本文复制引用
马艳萍,邹萍,肖娟,黄士昂..Caspase-3在脐血CD34+造血干/祖细胞中的表达及其意义[J].中德临床肿瘤学杂志(英文版),2003,2(3):166-168,3.基金项目
The study was supported by a grant from the National Natural Science Foundation of China (No. 39928010) （No. 39928010）
