生物技术通报Issue(7)：166-170,5.
β-甘露聚糖酶产生菌的分离、鉴定及产β-甘露聚糖酶最适条件的研究
Studies of the Isolation and Identification of Strain with β-mannanase and Its Enzymology Properties
摘要
Abstract
Two strains which produced β-mannanase were isolated from soil by enriching and isolating. A strain with the higher enzyme activity of 50.37 U/ml was obtained after the enzyme activity assay. The strain was identified as Pseudomonas sp. by morphological and physiological characteristics analysis and 16S rDNA BLAST. The optimal conditions for producing β-mannanase by the strain were determined 1 g/l00 ml maizena, 2 g/100 ml konjak powder, pH6.0 and the optimal temperature was 32 ℃, under which the activity of β-mannanase was up to 185.37 U/ml,3.68 times as before. The strain which rapidly produced β-mannanase showed the highest β-mannanase activity after culturing for 12 h. The study of the enzymatic character of the β-mannanase revealed that it was the acidic β-mannanase, and the optimal condition of the enzyme was pH4.0 and 60℃, respectively. The activity of β-mannanase was stable at pH4.0～6.0 and 40～55℃.关键词
β-甘露聚糖酶/假单孢菌/分离/鉴定/酶活性Key words
β-mannanase Pseudomonas sp. Isolation Identification Activity分类
生物科学引用本文复制引用
黄俊丽,包凌霞,王贵学..β-甘露聚糖酶产生菌的分离、鉴定及产β-甘露聚糖酶最适条件的研究[J].生物技术通报,2009,(7):166-170,5.基金项目
重庆市科技计划项目(2007AC1060),重庆大学重点实验室对本科生开放创新基金 （2007AC1060）
