首页|期刊导航|中华医学杂志（英文版）|Inhibition of proliferation and transforming growth factor B3 protein expression by peroxisome proliferators-activated receptor γ ligands in human uterine leiomyoma cells
Inhibition of proliferation and transforming growth factor B3 protein expression by peroxisome proliferators-activated receptor γ ligands in human uterine leiomyoma cells
ZHANG Chun-hua ZHANG Yao-jing WANG Ying-ying ZHANG Hui WEN Ze-qing LI Jian-feng LI Chang-zhong SHI Min YANG Gui-wen LAN Shou-min ZHU Yong WANG Fei
中华医学杂志（英文版）2008，Vol.121Issue(2)：166-171,6.
中华医学杂志（英文版）2008，Vol.121Issue(2)：166-171,6.
Inhibition of proliferation and transforming growth factor B3 protein expression by peroxisome proliferators-activated receptor γ ligands in human uterine leiomyoma cells
Inhibition of proliferation and transforming growth factor B3 protein expression by peroxisome proliferators-activated receptor γ ligands in human uterine leiomyoma cells
摘要
Abstract
Background Rosiglitazone is known as the most potent and specific peroxisome proliferators-activated receptor γ (PPAR-γ)ligand.It has potentially far-reaching effects on pathophysiological processes,from cancer to atherosclerosis and diabetes.However,it is not clear whether rosifllitazone affects the protein expression of transforming growth factor β3(TGF-β3)and lhe cell proliferation in human uterine leiomyoma cells in vitro.Methods Human uterine leiomyoma tissues were dissected and cultured.Cells were divided into 5 groups:one control group and other four groups with different concentrations of rosiglitazone(1O-7,10-8,10-9 and 10-10 mol/L).Cells were cultured for 72 hours in serum-free Dulbecco's modified Eagle's medium.MTT reduction assay was used to detect the cell proliferation.Reverse transcription polymerase chain reaction(RT-PCR)was used to detect the mRNA expression of PPAR-γ and TGF-β3.Immunofluorescence staining was used to detect the expressions of PPAR-γand TGF-β3 proteins.Results MTT reduction assay indicated that the treatment with rosiglitazone(from 10-7 to 10-9 mol/L) resulted in an inhibition of the cell growths after 72 hours(P＜0.01).RT-PCR analysis revealed that 10-7 mol/L rosiglitazone significantly affected the gene expression at 72-hour:PPAR-γ mRNA expression was up-regulated and TGF-β3 mRNA was down-regulated and rosiglitazone at the concentration of 10-7mol/L affected these most effectively(P＜0.01).Immunofluorescence staining demonstrated that treatment with 10-7mol/L rosifllitazone resulted in the significant changes of PPAR-γ and TGF-β3 protein expressions compared with the other treatment groups and the control group at 72-hour(P＜0.01).All the effects of rosiglitazone on uterine leiomyoma cells were dose-and time-dependent in vitro.Conclusions The present study demonstrates that the PPAR-γ activator,rosiglitazone,inhibits the cell proliferation partly through the regulations of PPAR-γ and TGF-β3 expressions.The cross-talk between the signal pathways of PPAR-γ and TGF-β3 may be jnvolved in the process.关键词
uterus/leiomyoma/peroxisome proliferators-activated receptor γ/transforming growth factor β3Key words
uterus/leiomyoma/peroxisome proliferators-activated receptor γ/transforming growth factor β3分类
医药卫生引用本文复制引用
ZHANG Chun-hua,ZHANG Yao-jing,WANG Ying-ying,ZHANG Hui,WEN Ze-qing,LI Jian-feng,LI Chang-zhong,SHI Min,YANG Gui-wen,LAN Shou-min,ZHU Yong,WANG Fei..Inhibition of proliferation and transforming growth factor B3 protein expression by peroxisome proliferators-activated receptor γ ligands in human uterine leiomyoma cells[J].中华医学杂志（英文版）,2008,121(2):166-171,6.基金项目
This work was supported by the Natural Science Foundation of Shandong Province(No.Y2006C67). （No.Y2006C67）
